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RabMAb

Anti-HDAC2抗体[EPR5001] (ab124974)

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概述

  • 产品名称
    Anti-HDAC2抗体[EPR5001]
    参阅全部 HDAC2 一抗
  • 描述
    兔单克隆抗体[EPR5001] to HDAC2
  • 经测试应用
    适用于: WB, IP, ICC, ChIPmore details
    不适用于: Flow Cyt or IHC-P
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues on the N-terminus in Human HDAC2 protein (Q92769).

  • 阳性对照
    • WB: Wild-type HAP1, HeLa, A431, SH-SY5Y, and Jurkat cell lysates. ICC/IF: Wild-type HAP1 cells.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

应用

Our Abpromise guarantee covers the use of ab124974 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/50000. Predicted molecular weight: 55 kDa.
IP 1/10 - 1/100.
ICC Use a concentration of 0.5 µg/ml.
ChIP Use 10 µg for 25 µg of chromatin.

Please note product formulation is not optimised for ChIP application
  • 应用说明
    Is unsuitable for Flow Cyt or IHC-P.

    • 靶标

    • 功能
      Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.
      Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Interacts in the late S-phase of DNA-replication with DNMT1 in the other transcriptional repressor complex composed of DNMT1, DMAP1, PCNA, CAF1. Deacetylates TSHZ3 and regulates its transcriptional repressor activity.
    • 组织特异性
      Widely expressed; lower levels in brain and lung.
    • 序列相似性
      Belongs to the histone deacetylase family. HD type 1 subfamily.
    • 翻译后修饰
      S-nitrosylated by GAPDH. In neurons, S-Nitrosylation at Cys-262 and Cys-274 does not affect the enzyme activity but abolishes chromatin-binding, leading to increases acetylation of histones and activate genes that are associated with neuronal development. In embryonic cortical neurons, S-Nitrosylation regulates dendritic growth and branching.
    • 细胞定位
      Nucleus.
    • Information by UniProt
    • 数据库链接
      see all
    • 别名
      • D10Wsu179e antibody
      • HD 2 antibody
      • HD2 antibody
      • HDAC 2 antibody
      • Hdac2 antibody
      • HDAC2_HUMAN antibody
      • Histone deacetylase 2 (HD2) antibody
      • Histone deacetylase 2 antibody
      • OTTHUMP00000017046 antibody
      • OTTHUMP00000227077 antibody
      • OTTHUMP00000227078 antibody
      • RPD3 antibody
      • transcriptional regulator homolog RPD3 antibody
      • YAF1 antibody
      • YY1 associated factor 1 antibody
      • YY1 transcription factor binding protein antibody
      • Yy1bp antibody
      see all

    Anti-HDAC2 antibody [EPR5001] 图像

    • Western blot - Anti-HDAC2 antibody [EPR5001] (ab124974)
      Western blot - Anti-HDAC2 antibody [EPR5001] (ab124974)


      Predicted band size : 55 kDa

      Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: HDAC2 knockout HAP1 whole cell lysate (20 µg)
      Lane 3: A431 whole cell lysate (20 µg)
      Lane 4: Hela whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab124974 observed at 60 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab124974 was shown to recognize HDAC2 when HDAC2 knockout samples were used, along with additional cross-reactive bands. Wild-type and HDAC2 knockout samples were subjected to SDS-PAGE.  ab124974 and ab8245 (Mouse anti GAPDH loading control) were but diluted at 1/10000 and incubated overnight at 4°C. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

       

    • Immunocytochemistry/ Immunofluorescence - Anti-HDAC2 antibody [EPR5001] (ab124974)
      Immunocytochemistry/ Immunofluorescence - Anti-HDAC2 antibody [EPR5001] (ab124974)

      ab124974 staining HDAC2 in wild-type HAP1 cells (top panel) and HDAC2 knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab124974 at 0.5μg/ml and ab195889 at 1/250 dilution (shown in pseudocolour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Western blot - Anti-HDAC2 antibody [EPR5001] (ab124974)
      Western blot - Anti-HDAC2 antibody [EPR5001] (ab124974)
      All lanes : Anti-HDAC2 antibody [EPR5001] (ab124974) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : SH-SY5Y cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 55 kDa
    • ChIP - Anti-HDAC2 antibody [EPR5001] (ab124974)
      ChIP - Anti-HDAC2 antibody [EPR5001] (ab124974)

      Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 10µg of ab124974 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach). Primers and probes are located in the first kb of the transcribed region.

    Anti-HDAC2 antibody [EPR5001] (ab124974)参考文献

    This product has been referenced in:
    • Lin MY  et al. Redirection of Epithelial Immune Responses by Short-Chain Fatty Acids through Inhibition of Histone Deacetylases. Front Immunol 6:554 (2015). WB ; Human . Read more (PubMed: 26579129) »

    See 1 Publication for this product

    发表研究结果有使用 ab124974？请让我们知道，以便我们可以引用本数据表中的参考文章。

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    3 Abreviews or Q&A

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    Western blot abreview for Anti-HDAC2 antibody [EPR5001]

     Average
    Abreviews
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Brain)
    Gel Running Conditions
    Reduced Denaturing (10% Gel)
    Loading amount
    20 µg
    Specification
    Brain
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
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    Abcam user community

    Verified customer

    提交于 Mar 28 2017

    Western blot abreview for Anti-HDAC2 antibody [EPR5001]

     Excellent
    Abreviews
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (Brain)
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Loading amount
    12 µg
    Specification
    Brain
    Blocking step
    BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More
    Username

    Abcam user community

    Verified customer

    提交于 Jun 25 2015

    Western blot abreview for Anti-HDAC2 antibody [EPR5001]

     Excellent
    Abreviews
    Application
    Western blot
    Sample
    Monkey Cell lysate - whole cell (Brain)
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Loading amount
    12 µg
    Specification
    Brain
    Blocking step
    Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    提交于 May 29 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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