概述

  • 产品名称Anti-HDAC2抗体
    参阅全部 HDAC2 一抗
  • 描述
    兔多克隆抗体to HDAC2
  • 特异性This antibody detects endogenous levels of total HDAC2 protein.
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    The antiserum was produced against synthesized non-phosphopeptide derived from human HDAC2 around the phosphorylation site of Serine 394.

性能

应用

Our Abpromise guarantee covers the use of ab39669 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 55 kDa.
ELISA 1/1000.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.
    Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Interacts in the late S-phase of DNA-replication with DNMT1 in the other transcriptional repressor complex composed of DNMT1, DMAP1, PCNA, CAF1. Deacetylates TSHZ3 and regulates its transcriptional repressor activity.
  • 组织特异性Widely expressed; lower levels in brain and lung.
  • 序列相似性Belongs to the histone deacetylase family. HD type 1 subfamily.
  • 翻译后修饰S-nitrosylated by GAPDH. In neurons, S-Nitrosylation at Cys-262 and Cys-274 does not affect the enzyme activity but abolishes chromatin-binding, leading to increases acetylation of histones and activate genes that are associated with neuronal development. In embryonic cortical neurons, S-Nitrosylation regulates dendritic growth and branching.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • D10Wsu179e antibody
    • HD 2 antibody
    • HD2 antibody
    • HDAC 2 antibody
    • Hdac2 antibody
    • HDAC2_HUMAN antibody
    • Histone deacetylase 2 (HD2) antibody
    • Histone deacetylase 2 antibody
    • OTTHUMP00000017046 antibody
    • OTTHUMP00000227077 antibody
    • OTTHUMP00000227078 antibody
    • RPD3 antibody
    • transcriptional regulator homolog RPD3 antibody
    • YAF1 antibody
    • YY1 associated factor 1 antibody
    • YY1 transcription factor binding protein antibody
    • Yy1bp antibody
    see all

Anti-HDAC2 antibody 图像



  • Predicted band size : 55 kDa
    Western blot analysis using HDAC2 antibody (ab39669)

    Lane 1: Extracts from HeLa cells;

    Lane 2: Extracts from 293 cells;

    Lane 3: Extracts from 293 cells using ab39669 preincubated with synthesized peptide.

  • Ab39669 staining human HDAC2 in human breast carcinoma by immunohistochemistry using paraffin embedded tissue.

    Left: Untreated.

    Right: Treated with synthesized peptide.

Anti-HDAC2 antibody (ab39669)参考文献

This product has been referenced in:
  • Benard A  et al. Nuclear expression of histone deacetylases and their histone modifications predicts clinical outcome in colorectal cancer. Histopathology 66:270-82 (2015). IHC-P ; Human . Read more (PubMed: 25307864) »
  • Derr RS  et al. High nuclear expression levels of histone-modifying enzymes LSD1, HDAC2 and SIRT1 in tumor cells correlate with decreased survival and increased relapse in breast cancer patients. BMC Cancer 14:604 (2014). IHC-P ; Human . Read more (PubMed: 25139823) »

See all 2 Publications for this product

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