概述

  • 产品名称Anti-HDAC2抗体
    参阅全部 HDAC2 一抗
  • 描述
    兔多克隆抗体to HDAC2
  • 经测试应用适用于: IHC-Fr, IHC-P, ICC/IF, WB, IPmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, African Green Monkey
    不与反应: Chicken
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human HDAC2.

    (Peptide available as ab16200.)

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: Hela; A431; Jurkat; HEK293 and Hela Nuclear lysate.

性能

应用

Our Abpromise guarantee covers the use of ab16032 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr 1/500.
IHC-P 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 55.3 kDa).
IP Use at an assay dependent concentration.

靶标

  • 功能Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.
    Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Interacts in the late S-phase of DNA-replication with DNMT1 in the other transcriptional repressor complex composed of DNMT1, DMAP1, PCNA, CAF1. Deacetylates TSHZ3 and regulates its transcriptional repressor activity.
  • 组织特异性Widely expressed; lower levels in brain and lung.
  • 序列相似性Belongs to the histone deacetylase family. HD type 1 subfamily.
  • 翻译后修饰S-nitrosylated by GAPDH. In neurons, S-Nitrosylation at Cys-262 and Cys-274 does not affect the enzyme activity but abolishes chromatin-binding, leading to increases acetylation of histones and activate genes that are associated with neuronal development. In embryonic cortical neurons, S-Nitrosylation regulates dendritic growth and branching.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • D10Wsu179e antibody
    • HD 2 antibody
    • HD2 antibody
    • HDAC 2 antibody
    • Hdac2 antibody
    • HDAC2_HUMAN antibody
    • Histone deacetylase 2 (HD2) antibody
    • Histone deacetylase 2 antibody
    • OTTHUMP00000017046 antibody
    • OTTHUMP00000227077 antibody
    • OTTHUMP00000227078 antibody
    • RPD3 antibody
    • transcriptional regulator homolog RPD3 antibody
    • YAF1 antibody
    • YY1 associated factor 1 antibody
    • YY1 transcription factor binding protein antibody
    • Yy1bp antibody
    see all

Anti-HDAC2 antibody 图像

  • ICC/IF image of ab16032 stained HeLa cells. The cells were 100% methanol fixed (5 min) then permeabilised using 0.1% PBS-Triton and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to further permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab16032 at 5µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • All lanes : Anti-HDAC2 antibody (ab16032) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 4 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 55.3 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute
  • HDAC2 was immunoprecipitated using 0.5mg Hela whole cell extract, 5ug of Rabbit polyclonal to HDAC2 and 50µl of protein G magnetic beads (lane 1). The antibody was incubated with the Protein G beads for 10min under agitation. No antibody was added to the control (lane 2). Hela whole cell extractdiluted in RIPA buffer was added to each sample and incubated for 10min under agitation. Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab16032. Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697). Band: 60ka: HDAC2.
  • ab16032 (1/250) staining HDAC2 in paraffin-embedded Human tonsil tissue.  Tissue underwent fixation in formaldehyde, peroxidase blocking, protein blocking and heat mediated antigen retrieval.  The secondary antibody was goat anti rabbit/mouse conjugated to HRP.  For further experimental details please refer to abreview.

    See Abreview

  • IHC-Fr image of HDAC2 (ab16032) staining on Rat spinal cord. The sections required antigen retrieval with sodium citrate buffer was necessary to obtain full signal strength (sodium citrate 10mM, pH6)

    See Abreview

  • All lanes : Anti-HDAC2 antibody (ab16032) at 0.4 µg/ml

    Lane 1 : Mouse 3T3 lysate
    Lane 2 : Rat liver lysate
    Lane 3 : Chicken liver lysate

    Lysates/proteins at 20 µg/ml per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 55.3 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
    ab16032 cross-reacts with Mouse 3T3 cells but shows no cross-reactivity against either Rat liver or Chicken cell lysates.

Anti-HDAC2 antibody (ab16032)参考文献

This product has been referenced in:
  • Yao ZG  et al. Regional and cell-type specific distribution of HDAC2 in the adult mouse brain. Brain Struct Funct 218:563-73 (2013). Mouse . Read more (PubMed: 22532304) »
  • Lee TK  et al. An N-terminal truncated carboxypeptidase E splice isoform induces tumor growth and is a biomarker for predicting future metastasis in human cancers. J Clin Invest : (2011). WB ; Human . Read more (PubMed: 21285511) »

See all 7 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (human breast cancer tissue sections)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: pH 6.0 citric acid
Permeabilization Yes - tween-20
Specification human breast cancer tissue sections
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Nov 17 2015

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (primary culture cell of human breast carcinoma tis)
Permeabilization Yes - tween-20
Specification primary culture cell of human breast carcinoma tis
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative Paraformaldehyde
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Abcam user community

Verified customer

提交于 Nov 17 2015

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (hippocampus)
Specification hippocampus
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Miss. Sung-Eun WANG

Verified customer

提交于 Apr 17 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 22 µg
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Sample Mouse Cell lysate - whole cell (293T, MEL cell)
Specification 293T, MEL cell
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jul 03 2013

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Sample African Green Monkey Cell (Cos7 Monkey Kidney Fibroblast)
Specification Cos7 Monkey Kidney Fibroblast
Permeabilization Yes - .3% Triton X100
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jun 13 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (spinal cord)
Specification spinal cord
Fixative Paraformaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Sep 06 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Tonsil)
Specification Tonsil
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Target Retrieval Solution
Permeabilization No
Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

提交于 Mar 25 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Human colon adenocarcinoma cells)
Loading amount 200000 cells
Specification Human colon adenocarcinoma cells
Treatment transfected with plasmids (irrelevant to HDAC2)
Gel Running Conditions Reduced Denaturing (12% gel)
Blocking step Milk as blocking agent for 40 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Ms. Johanna Smith

Verified customer

提交于 Jul 11 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (various cell types - cell lines and human primary)
Loading amount 15 µg
Specification various cell types - cell lines and human primary
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 25°C
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Prof. Paul Townsend

Verified customer

提交于 May 01 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"