概述

  • 产品名称Anti-HDAC11抗体
    参阅全部 HDAC11 一抗
  • 描述
    兔多克隆抗体to HDAC11
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Cow, Rhesus monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human HDAC11.

    (Peptide available as ab98895.)

  • 阳性对照
    • This antibody gave a positive signal in Human Skeletal Muscle Tissue Lysate as well as HeLa and NIH3T3 Whole Cell Lysates.

性能

应用

Our Abpromise guarantee covers the use of ab87765 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).

靶标

  • 功能Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.
  • 组织特异性Weakly expressed in most tissues. Strongly expressed in brain, heart, skeletal muscle, kidney and testis.
  • 序列相似性Belongs to the histone deacetylase family.
  • 细胞定位Nucleus. Predominantly nuclear.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ22237 antibody
    • HD 11 antibody
    • HD11 antibody
    • HDA11_HUMAN antibody
    • HDAC 11 antibody
    • HDAC11 antibody
    • Histone deacetylase 11 antibody
    see all

Anti-HDAC11 antibody 图像

  • All lanes : Anti-HDAC11 antibody (ab87765) at 1 µg/ml

    Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 37 kDa
    Observed band size : 37 kDa
    Additional bands at : 52 kDa,60 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • Anti-HDAC11 antibody (ab87765) at 1 µg/ml + Recombinant Human HDAC11 protein (ab101113) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute
  • Anti-HDAC11 antibody (ab87765) at 1 µg/ml + NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 37 kDa
    Observed band size : 37 kDa


    Exposure time : 20 minutes

Anti-HDAC11 antibody (ab87765)参考文献

ab87765 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab87765.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"