概述

  • 产品名称
  • 描述
    兔多克隆抗体to HDAC11
  • 特异性
    ab18973 recognises 39 kDa HDAC-11 of human and mouse origin. It also reacts with rat weakly.
  • 经测试应用
    适用于: IHC-FoFr, WB, ICC/IF, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide, corresponding to amino acids surrounding 269 of Human HDAC 11

  • 阳性对照
    • Jurkat cell lysate, Mouse intestine tissue lysate.

性能

应用

Our Abpromise guarantee covers the use of ab18973 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr Use at an assay dependent concentration. PubMed: 17893925
WB Use a concentration of 1 - 4 µg/ml. Predicted molecular weight: 39 kDa.
ICC/IF 1/100. PubMed: 23469282
IHC-Fr Use at an assay dependent concentration. PubMed: 25011550

靶标

  • 功能
    Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.
  • 组织特异性
    Weakly expressed in most tissues. Strongly expressed in brain, heart, skeletal muscle, kidney and testis.
  • 序列相似性
    Belongs to the histone deacetylase family.
  • 细胞定位
    Nucleus. Predominantly nuclear.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ22237 antibody
    • HD 11 antibody
    • HD11 antibody
    • HDA11_HUMAN antibody
    • HDAC 11 antibody
    • HDAC11 antibody
    • Histone deacetylase 11 antibody
    see all

图片

  • All lanes : Anti-HDAC11 antibody (ab18973) at 2.5 µg/ml

    Lane 1 : Jurkat cell lysate
    Lane 2 : Mouse intestine tissue lysate


    Predicted band size : 39 kDa

文献

This product has been referenced in:
  • Liu J  et al. Both HDAC5 and HDAC6 are required for the proliferation and metastasis of melanoma cells. J Transl Med 14:7 (2016). Human . Read more (PubMed: 26747087) »
  • Tiwari S  et al. Histone deacetylase expression patterns in developing murine optic nerve. BMC Dev Biol 14:30 (2014). WB, IHC-Fr ; Mouse . Read more (PubMed: 25011550) »

See all 9 Publications for this product

客户评价及客户问答

Application
Flow Cytometry
Fixation
Paraformaldehyde
Permeabilization
Yes - BD permeabilization buffer
Sample
Mouse Cell (Mouse thymus tissue, thymocytes)
Specification
Mouse thymus tissue, thymocytes
Gating Strategy
CD45 positives
Preparation
Cell harvesting/tissue preparation method: Digestion with Collagenase D
Sample buffer: PBS 2% FCS
Username

Mr. Sanjay Gawade

Verified customer

提交于 Jan 26 2015

Thank you for your reply and for the nice feedback. I am happy the information was helpful to you.

I have issued a credit note for the value of the antibody ab18973.

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Thank you for your reply.

Any of the alternatives anti HDAC11 validated in WB with mouse samples should work as stated on the datasheet. There are 4 http://www.abcam.com/index.html?pageconfig=searchresults&search=HDAC11&pt=1&sk=re...

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Thank you very much for taking the time to complete our questionnaire and sending the images.

I am sorry to hear you have been experiencing problems with one of our antibodies. The quality of our products is important to us and I would like t...

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Thank you for your enquiry. Unfortunately I do not have access to the Bradbury publication. However, I can tell you that ab18973; Rabbit polyclonal to HDAC11 was not in our catalogue until 02 Nov 05. Therefore given the date of publication I consid...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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