概述

  • 产品名称Anti-HDAC10抗体
    参阅全部 HDAC10 一抗
  • 描述
    兔多克隆抗体to HDAC10
  • 特异性ab53096detects endogenous levels of total HDAC10 protein.
  • 经测试应用适用于: ICC/IF, WB, IHC-P, ELISA, IHC-FoFrmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (Human)

  • 阳性对照
    • IHC-P: Human lung carcinoma tissue WB: Cos7 cell extract

性能

应用

Our Abpromise guarantee covers the use of ab53096 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
WB 1/300 - 1/1000. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/5000.
IHC-FoFr Use at an assay dependent concentration. PubMed: 21526203

靶标

  • 功能Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.
  • 组织特异性Ubiquitous. High expression in liver, spleen, pancreas and kidney.
  • 序列相似性Belongs to the histone deacetylase family. HD type 2 subfamily.
  • 细胞定位Cytoplasm. Nucleus. Excluded from the nucleoli.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZP761B039 antibody
    • HD 10 antibody
    • HD10 antibody
    • HDA10_HUMAN antibody
    • HDAC 10 antibody
    • Hdac10 antibody
    • Histone deacetylase 10 antibody
    • MGC149722 antibody
    • OTTHUMP00000028555 antibody
    see all

Anti-HDAC10 antibody 图像

  • ab53096, at 1/50 dilution, staining HDAC10 in paraffin embedded human lung carcinoma tissue by Immunohistochemistry in the absence (left image) or presence (right image) of the immunising peptide.
  • All lanes : Anti-HDAC10 antibody (ab53096) at 1/300 dilution

    Lane 1 : Cos7 cell extract
    Lane 2 : Cos7 cell extract with peptide


    Predicted band size : 71 kDa
    Observed band size : 71 kDa
  • ICC/IF image of ab53096 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53096, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-HDAC10 antibody (ab53096)参考文献

This product has been referenced in:
  • Takase K  et al. Monoaminergic and neuropeptidergic neurons have distinct expression profiles of histone deacetylases. PLoS One 8:e58473 (2013). ICC/IF ; Mouse . Read more (PubMed: 23469282) »
  • Funato H  et al. Fasting and high-fat diet alter histone deacetylase expression in the medial hypothalamus. PLoS One 6:e18950 (2011). IHC-FoFr ; Mouse . Read more (PubMed: 21526203) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (liver)
Loading amount 50 µg
Specification liver
Treatment caloric restriction
Gel Running Conditions Reduced Denaturing (7.5%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Jul 07 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"