HDAC1 Sumoylation Assay试剂盒(ab115132)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 3 hr
- Sample type: Cell culture extracts, Tissue Extracts
概述
-
产品名称
HDAC1 Sumoylation Assay试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture extracts, Tissue Extracts -
检测类型
Quantitative -
检测时间
3h 00m -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Mammals -
产品概述
Sumoylation is a post-transcriptional modification involved in various cellular processes such as nuclear-cytoplasmic transport, transcription regulation and progression through the cell cycle. There are 3 confirmed SUMO isoforms in humans: SUMO-1, SUMO-2 and SUMO-3. SUMO-2/-3 show a high degree of similarity to each other and are distinct from SUMO-1. Sumoylation is directed by an enzymatic cascade analogous to that involved in ubiquitination. Sumoylation of target proteins in vivo has been shown to cause a number of different outcomes, including altered localization and binding partners.
HDAC1 is a class I histone deacetylase that plays a critical role in transcriptional repression of gene expression and has been shown to be an integral component of multiprotein co-repressor complexes. It has been observed that an important mechanism of HDAC1 activity regulation is sumoylation, which acts by potentiating HDAC1 activity.
Abcam's HDAC1 Sumoylation Assay Kit (ab115132) allows the user to measure in vivo HDAC1 sumoylation. The kit is ready-to-use and provides all the essential components needed for measuring in vivo HDAC1 sumoylation from multiple mammalian cells/tissues extracts.
-
平台
Microplate reader
性能
-
存放说明
Please refer to protocols. -
组件 48 tests 96 tests 10X Wash Buffer 1 x 25ml 1 x 50ml 8-Well Assay Strips (with Frame) 6 units 12 units 8-Well Control Strips 2 units 3 units Color Development Solution 1 x 6ml 1 x 12ml Signal Enhancer 1 x 120µl 1 x 240µl Signal Report Solution 1 x 10µl 1 x 20µl Stop Solution 1 x 3ml 1 x 6ml Sumo Antibody, 1µg/µL 1 x 5µl 1 x 10µl Sumo Assay Buffer 1 x 2ml 1 x 4ml Sumo Protein, 1 µg/µL 1 x 6µl 1 x 12µl -
研究领域
-
别名
- GON 10
- HD1
- HDAC 1
see all -
数据库链接
- Entrez Gene: 3065 Human
- Entrez Gene: 433759 Mouse
- Entrez Gene: 297893 Rat
- Omim: 601241 Human
- SwissProt: Q13547 Human
- SwissProt: O09106 Mouse
- SwissProt: Q4QQW4 Rat
相关产品
-
Related Products
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab115132 尚未被引用在任何文献中。