HDAC1 overexpression 293T裂解液(全细胞) (ab94165)

概述

  • 产品名称HDAC1 overexpression 293T裂解液(全细胞)
  • 描述
    HDAC1 overexpression裂解液
  • 常规说明ab94165 is a 293T cell transfected lysate in which Human HDAC1 has been transiently over-expressed using a pCMV-HDAC1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • 经测试应用适用于: WBmore details

性能

  • 背景Function: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B. PTM: Sumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1. Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes. Ubiquitinated by CHFR, leading to its degradation by the proteasome. Similarity: Belongs to the histone deacetylase family. HD type 1 subfamily. Tissue specificity: Ubiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain.

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应用

Our Abpromise guarantee covers the use of ab94165 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution.

HDAC1 overexpression 293T lysate (whole cell) 图像

  • ab94165 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-HDAC1 antibody (ab55094) at 1/500 dilution

    Lane 1 : HDAC1 overexpression 293T lysate (whole cell) (ab94165)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

HDAC1 overexpression 293T lysate (whole cell) (ab94165)参考文献

ab94165 has not yet been referenced specifically in any publications.

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