Anti-HDAC1抗体,prediluted (ab15316)


  • 产品名称
    参阅全部 HDAC1 一抗
  • 描述
    兔多克隆抗体to HDAC1,prediluted
  • 宿主
  • 经测试应用
    适用于: ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide (unfortunately, the amino acid sequence is considered to be commercially sensitive) (Human) (C terminal).

  • 阳性对照
    • NIH/3T3 cells or Tonsil This antibody gave a positive result when used in the following formaldehydel fixed cell lines: HepG2.



Our Abpromise guarantee covers the use of ab15316 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
  • 应用说明
    IHC-P: Use at an assay dependent dilution. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B.
    • 组织特异性
      Ubiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain.
    • 序列相似性
      Belongs to the histone deacetylase family. HD type 1 subfamily.
    • 翻译后修饰
      Sumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1.
      Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes.
      Ubiquitinated by CHFR, leading to its degradation by the proteasome.
    • 细胞定位
    • Information by UniProt
    • 数据库链接
    • 别名
      • DKFZp686H12203 antibody
      • GON 10 antibody
      • HD1 antibody
      • HDAC 1 antibody
      • HDAC1 antibody
      • HDAC1_HUMAN antibody
      • Histone deacetylase 1 antibody
      • Reduced potassium dependency yeast homolog like 1 antibody
      • RPD3 antibody
      • RPD3L1 antibody
      see all


    • ab15316 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab15316 at 1/50 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • ab15316 staining HDAC1 in breast carcinoma by Immunohistochemistry (FFPE-sections).
    • Immunohistochemical analysis of Human vulvar squamous cell cancer, staining HDAC1 with ab15316 at 1/2 dilution.
    • Immunohistochemical analysis of Human vulvar intraepithelial neoplasia, staining HDAC1 with ab15316 at 1/2 dilution.


    This product has been referenced in:
    • Peper JK  et al. HLA ligandomics identifies histone deacetylase 1 as target for ovarian cancer immunotherapy. Oncoimmunology 5:e1065369 (2016). IHC . Read more (PubMed: 27467910) »
    • Samartzis EP  et al. The Expression of Histone Deacetylase 1, But Not Other Class I Histone Deacetylases, Is Significantly Increased in Endometriosis. Reprod Sci N/A:N/A (2013). Human . Read more (PubMed: 23690335) »

    See all 7 Publications for this product


    Thank you for your message. I can confirm that the alignment of the immunogen for ab15316 HDAC1 antibody with HDAC 2,3,6,and 10 is as follows: HDAC2 54.50% HDAC3 35.70% HDAC6 0% HDAC10 0% These alignments are very low and so I would s...

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