Anti-HDAC1抗体[mAbcam 68436] -核Loading Control (ab68436)

概述

  • 产品名称Anti-HDAC1抗体[mAbcam 68436] -核Loading Control
    参阅全部 HDAC1 一抗
  • 描述
    小鼠单克隆抗体[mAbcam 68436] to HDAC1 -核Loading Control
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat, Cow, Human, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human HDAC1.

    (Peptide available as ab20434.)

  • 阳性对照
    • This antibody gave a positive signal in IOUD2 Whole Cell Lysate.

性能

应用

Our Abpromise guarantee covers the use of ab68436 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 5 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 55 kDa).

靶标

  • 功能Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B.
  • 组织特异性Ubiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain.
  • 序列相似性Belongs to the histone deacetylase family. HD type 1 subfamily.
  • 翻译后修饰Sumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1.
    Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes.
    Ubiquitinated by CHFR, leading to its degradation by the proteasome.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp686H12203 antibody
    • GON 10 antibody
    • HD1 antibody
    • HDAC 1 antibody
    • HDAC1 antibody
    • HDAC1_HUMAN antibody
    • Histone deacetylase 1 antibody
    • Reduced potassium dependency yeast homolog like 1 antibody
    • RPD3 antibody
    • RPD3L1 antibody
    see all

Anti-HDAC1 antibody [mAbcam 68436] - Nuclear Loading Control 图像

  • Anti-HDAC1 antibody [mAbcam 68436] - Nuclear Loading Control (ab68436) at 5 µg/ml + IOUD2 Whole Cell Lysate at 20 µg

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 55 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

Anti-HDAC1 antibody [mAbcam 68436] - Nuclear Loading Control (ab68436)参考文献

ab68436 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Spinal cord)
Specification Spinal cord
Fixative Paraformaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
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提交于 Aug 24 2010

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