Anti-HDAC1抗体[mAbcam 68436] -核Loading Control (ab68436)
Key features and details
- Mouse monoclonal [mAbcam 68436] to HDAC1 - Nuclear Loading Control
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
概述
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产品名称
Anti-HDAC1抗体[mAbcam 68436] -核Loading Control
参阅全部 HDAC1 一抗 -
描述
小鼠单克隆抗体[mAbcam 68436] to HDAC1 -核Loading Control -
宿主
Mouse -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse
预测可用于: Rat, Cow, Human, Orangutan -
免疫原
Synthetic peptide corresponding to Human HDAC1 aa 450 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab20434) -
阳性对照
- WB: IOUD2 Whole Cell Lysate.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
mAbcam 68436 -
骨髓瘤
Sp2/0-Ag14 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab68436于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 5 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 55 kDa).
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说明 |
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WB
Use a concentration of 5 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 55 kDa). |
靶标
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功能
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B. -
组织特异性
Ubiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain. -
序列相似性
Belongs to the histone deacetylase family. HD type 1 subfamily. -
翻译后修饰
Sumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1.
Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes.
Ubiquitinated by CHFR, leading to its degradation by the proteasome. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 404126 Cow
- Entrez Gene: 3065 Human
- Entrez Gene: 433759 Mouse
- Entrez Gene: 100172663 Orangutan
- Entrez Gene: 297893 Rat
- Omim: 601241 Human
- SwissProt: Q32PJ8 Cow
- SwissProt: Q13547 Human
see all -
别名
- DKFZp686H12203 antibody
- GON 10 antibody
- HD1 antibody
see all
图片
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Anti-HDAC1 antibody [mAbcam 68436] - Nuclear Loading Control (ab68436) at 5 µg/ml + IOUD2 Whole Cell Lysate at 20 µg
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 55 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
数据表及文件
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Datasheet download
文献 (2)
ab68436 被引用在 2 文献中.
- Asadian P et al. The expression profile of sterile alpha motif and histidine-aspartate domain-containing protein 1 (SAMHD1) in feline tissues. Vet Immunol Immunopathol 195:7-18 (2018). WB . PubMed: 29249320
- Remes Lenicov F et al. Prostaglandin E2 Antagonizes TGF-ß Actions During the Differentiation of Monocytes Into Dendritic Cells. Front Immunol 9:1441 (2018). PubMed: 29988364