概述

  • 产品名称
  • 描述
    兔多克隆抗体to HDAC1
  • 特异性
    ab53091 detects endogenous levels of total HDAC1 protein.
  • 经测试应用
    适用于: Flow Cyt, ICC, WB, IHC-P, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide derived from human HDAC1

  • 阳性对照
    • WB: NIH/3T3 cell extracts IHC-P: Human lung carcinoma tissue

性能

应用

Our Abpromise guarantee covers the use of ab53091 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
ICC Use at an assay dependent concentration.
WB 1/300 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 55 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/10000.
ICC/IF Use a concentration of 1 µg/ml.

靶标

  • 功能
    Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B.
  • 组织特异性
    Ubiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain.
  • 序列相似性
    Belongs to the histone deacetylase family. HD type 1 subfamily.
  • 翻译后修饰
    Sumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1.
    Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes.
    Ubiquitinated by CHFR, leading to its degradation by the proteasome.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp686H12203 antibody
    • GON 10 antibody
    • HD1 antibody
    • HDAC 1 antibody
    • HDAC1 antibody
    • HDAC1_HUMAN antibody
    • Histone deacetylase 1 antibody
    • Reduced potassium dependency yeast homolog like 1 antibody
    • RPD3 antibody
    • RPD3L1 antibody
    see all

图片

  • ICC/IF image of ab53091 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53091, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using ab53091 at 1/50 dilution, in the presence (right) or absence (left) of blocking peptide.
  • Immunocytochemistry/ Immunofluorescence analysis of mouse RAW 264.7 cells labeling HDAC1 with ab53091 at 1/150 dilution. The cells were fixed with paraformaldehyde and permeabilized with 0.1% Triton-X100 in 2% BSA for 15min. Blocking of the cells was done with 2% BSA for 1 hour at 4°C, followed by staining with ab53091 at 1/150 in 2% BSA in PBS for 16 hours at 4°C. An Alexa Fluor® 488 conjugated chicken anti-rabbit IgG (H+L) secondary antibody was used at 1/1000 dilution.

    See Abreview

  • All lanes : Anti-HDAC1 antibody (ab53091) at 1/300 dilution

    Lane 1 : NIH/3T3 cell extracts
    Lane 2 : NIH/3T3 cell extracts with blocking peptide


    Predicted band size : 55 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  • Flow Cytometry analysis of human mononuclear cell preparations labeling HDAC1 with ab53091 at 1/100 dilution (green). Cells were harvested using a apheresis preparation method. Cells were then fixed with paraformaldehyde and permeabilized with 0.1% Triton-X100 in 2% BSA for 15 min. An Alexa Fluor® 488 conjugated chicken anti-rabbit IgG (H+L) was used as the secondary antibody at 1/500 dilution. Blue - secondary only, red - unstained cells.

    See Abreview

  • All lanes : Anti-HDAC1 antibody (ab53091)

    Lane 1 : NIH/3T3 cell lysates
    Lane 2 : NIH/3T3 cell lysates blocked with synthesized peptide


    Predicted band size : 55 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labeling HDAC1 with ab53091. The image on the right is blocked with the synthesized peptide.

  • Immunocytochemistry/ Immunofluorescence analysis of NIH/3T3 cells labeling HDAC1 with ab53091. The image on the right is blocked with the synthesized peptide.

文献

This product has been referenced in:
  • Deng Q  et al. Androgen Receptor Localizes to Plasma Membrane by Binding to Caveolin-1 in Mouse Sertoli Cells. Int J Endocrinol 2017:3985916 (2017). WB, IP, IF ; Mouse . Read more (PubMed: 28642789) »
  • Wang Y  et al. Class I HDAC imaging using [ (3)H]CI-994 autoradiography. Epigenetics 8:756-64 (2013). Read more (PubMed: 23803584) »

See all 2 Publications for this product

客户评价及客户问答

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Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10% gel)
Sample
Mouse Tissue lysate - whole (hippocampus)
Specification
hippocampus
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Apr 16 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10% gel)
Sample
Rat Tissue lysate - whole (hippocampus)
Specification
hippocampus
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Miss. Sung-Eun WANG

Verified customer

提交于 Aug 30 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Mouse Cultured Cells (RAW 264.7)
Specification
RAW 264.7
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton-X100 in 2% BSA for 15min
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 4°C
Username

Dr. Mahesh Shivananjappa

Verified customer

提交于 Jun 20 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Monocytes)
Specification
Monocytes
Fixative
BD Cytofix/Cytoperm 20 min
Permeabilization
Yes - BD Cytofix/Cytoperm 20 min
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Dr. Mahesh Shivananjappa

Verified customer

提交于 Feb 06 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK 293)
Loading amount
30 µg
Specification
HEK 293
Gel Running Conditions
Reduced Denaturing (8-16% Biorad Criterion Gel with 18 wells)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Dr. Mahesh Shivananjappa

Verified customer

提交于 Dec 12 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (Mononuclear Cell Preparations)
Specification
Mononuclear Cell Preparations
Preparation
Cell harvesting/tissue preparation method: Apheresis
Sample buffer: PBS
Fixation
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton-X100 in 2% BSA for 15min
Gating Strategy
Monocytes Lymphocytes
Username

Dr. Mahesh Shivananjappa

Verified customer

提交于 Mar 31 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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