Anti-HDAC1抗体[1.T.9] - ChIP Grade (ab31263)

概述

  • 产品名称Anti-HDAC1抗体[1.T.9] - ChIP Grade
    参阅全部 HDAC1 一抗
  • 描述
    小鼠单克隆抗体[1.T.9] to HDAC1 - ChIP Grade
  • 经测试应用适用于: WB, ICC, IP, ICC/IF, IHC-FoFrmore details
  • 种属反应性
    与反应: Mouse, Cow, Human, African Green Monkey
    预测可用于: Rat, Dog, Chimpanzee
  • 免疫原

    Synthetic peptide:

    CEEKPEAKGVKEEVKLA

    conjugated to KLH, corresponding to amino acids 467-482 of Mouse HDAC1

  • 阳性对照
    • 3T3/A31 cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab31263 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 60 kDa.
ICC Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 22416134
IHC-FoFr 1/1000. PubMed: 19424149

靶标

  • 功能Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B.
  • 组织特异性Ubiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain.
  • 序列相似性Belongs to the histone deacetylase family. HD type 1 subfamily.
  • 翻译后修饰Sumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1.
    Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes.
    Ubiquitinated by CHFR, leading to its degradation by the proteasome.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp686H12203 antibody
    • GON 10 antibody
    • HD1 antibody
    • HDAC 1 antibody
    • HDAC1 antibody
    • HDAC1_HUMAN antibody
    • Histone deacetylase 1 antibody
    • Reduced potassium dependency yeast homolog like 1 antibody
    • RPD3 antibody
    • RPD3L1 antibody
    see all

Anti-HDAC1 antibody [1.T.9] - ChIP Grade 图像

  • ab31263 staining HDAC1 in COS7 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 2% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/200 in 2% BSA) for 1 hour at 25°C. A DyLight® 488-conjugated goat anti-mouse IgG polyclonal (1/100) was used as the secondary antibody.

    See Abreview

  • Anti-HDAC1 antibody [1.T.9] - ChIP Grade (ab31263) at 0.1 µg/ml + 3T3 cell lysate

    Predicted band size : 60 kDa

Anti-HDAC1 antibody [1.T.9] - ChIP Grade (ab31263)参考文献

This product has been referenced in:
  • Martínez FP & Tang Q Leucine zipper domain is required for Kaposi sarcoma-associated herpesvirus (KSHV) K-bZIP protein to interact with histone deacetylase and is important for KSHV replication. J Biol Chem 287:15622-34 (2012). WB, ICC/IF ; Human . Read more (PubMed: 22416134) »
  • Kurita M  et al. HDAC2 regulates atypical antipsychotic responses through the modulation of mGlu2 promoter activity. Nat Neurosci 15:1245-54 (2012). Read more (PubMed: 22864611) »

See all 8 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (esophagus)
Gel Running Conditions Non-reduced Non-Denaturing (Native)
Loading amount 40 µg
Specification esophagus
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
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提交于 Sep 05 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Brain)
Gel Running Conditions Reduced Denaturing (4-20%)
Loading amount 12 µg
Specification Brain
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Apr 01 2016

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Sample African Green Monkey Cell (COS7)
Specification COS7
Permeabilization Yes - Triton X100 0.3%
Fixative Paraformaldehyde
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提交于 Oct 20 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"