Anti-HCN4抗体[S114-10] (ab85023)

概述

  • 产品名称Anti-HCN4抗体[S114-10]
    参阅全部 HCN4 一抗
  • 描述
    小鼠单克隆抗体[S114-10] to HCN4
  • 特异性No cross reactivity against other HCNs.
  • 经测试应用适用于: IHC-Fr, WB, ICC/IF, IHC-P, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Rainbow Trout
  • 免疫原

    Fusion protein: PRGGLSPPGH SPGPPRTFPS APPRASGSHG SLLLPPASSP PPPQVPQRRG TPPLTPGRLT QDLKLISASQ PALPQDGAQT LRRASPHSSG, corresponding to cytoplasmic C terminal amino acids 1019-1108 of Rat HCN4 (Q9JKA7).

  • 阳性对照
    • COS cell lysate transiently transfected with HCN4.

性能

应用

Our Abpromise guarantee covers the use of ab85023 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use a concentration of 0.1 - 1 µg/ml.
WB Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 129 kDa.
ICC/IF Use a concentration of 1 - 10 µg/ml.
IHC-P Use a concentration of 0.1 - 1 µg/ml.
Flow Cyt Use 2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 相关性HCN4 is a member of the family of hyperpolarization activated and cyclic nucleotide gated (HCN) channels. HCN currents have been linked to pacemaker activity in the heart and brain, resting potential control, as well as neuronal plasticity. It has been shown that HCN4 channels function as receptors for sour taste, and are associated with pacemaker potential generation in the sinoatrial node.
  • 细胞定位Membrane; multi pass membrane protein.
  • 数据库链接
  • 别名
    • HCN 4 antibody
    • Hyperpolarization activated cyclic nucleotide gated potassium channel 4 antibody
    • Potassium/sodium hyperpolarization activated cyclic nucleotide gated channel 4 antibody

Anti-HCN4 antibody [S114-10] 图像

  • ab85023 staining HCN4 in human hippocampus tissue section by IHC-P. Bouin's fixed and paraffin-embedded tissue sections were used.

  • Overlay histogram showing SH-SY5Y cells stained with ab85023 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab85023, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-HCN4 antibody [S114-10] (ab85023)参考文献

This product has been referenced in:
  • Mitrofanova LB  et al. Evidence of specialized tissue in human interatrial septum: histological, immunohistochemical and ultrastructural findings. PLoS One 9:e113343 (2014). IHC-P ; Human . Read more (PubMed: 25412099) »
  • Grunow B  et al. In vitro developed spontaneously contracting cardiomyocytes from rainbow trout as a model system for human heart research. Cell Physiol Biochem 27:1-12 (2011). ICC/IF ; Rainbow Trout . Read more (PubMed: 21325816) »

See all 3 Publications for this product

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Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (hESC-derived cardiomyocyte)
Specification hESC-derived cardiomyocyte
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton X-100 in PBS
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
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提交于 Dec 17 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"