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Our Abpromise guarantee covers the use of ab69054 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 155 kDa (predicted molecular weight: 129 kDa).|
|ICC/IF||Use a concentration of 10 µg/ml.|
HCN4 contains a potential glycosylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted. This higher band has been described in the following publications: PMID: 14657344, PMID: 19471099.
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab69054 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
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