Anti-HCN2抗体[S71-37] (ab84817)

概述

  • 产品名称Anti-HCN2抗体[S71-37]
    参阅全部 HCN2 一抗
  • 描述
    小鼠单克隆抗体[S71-37] to HCN2
  • 特异性Does not cross react with HCN1.
  • 经测试应用适用于: IHC-P, IHC-Fr, WB, ICC/IF, IPmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Fusion protein, corresponding to cytoplasmic C terminal amino acids 761-863 of Rat HCN2

  • 阳性对照
    • Rat brain lysate.

性能

应用

Our Abpromise guarantee covers the use of ab84817 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 95 kDa.
ICC/IF Use a concentration of 1 - 10 µg/ml.
IP Use at an assay dependent concentration.

靶标

  • 功能Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Produces a large instantaneous current. Activated by cAMP. Modulated by intracellular chloride ions and pH; acidic pH shifts the activation to more negative voltages.
  • 组织特异性Highly expressed throughout the brain. Detected at low levels in heart.
  • 序列相似性Belongs to the potassium channel HCN family.
    Contains 1 cyclic nucleotide-binding domain.
  • 结构域The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BCNG-2 antibody
    • BCNG2 antibody
    • Brain cyclic nucleotide gated channel 2 antibody
    • Brain cyclic nucleotide-gated channel 2 antibody
    • HAC1 antibody
    • Hcn2 antibody
    • HCN2_HUMAN antibody
    • Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 antibody
    see all

Anti-HCN2 antibody [S71-37] 图像

  • Anti-HCN2 antibody [S71-37] (ab84817) at 1/1000 dilution + Rat brain membrane lysate

    Predicted band size : 95 kDa
  • ab84817 staining HCN2 in Mouse brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections).

  • ab84817 staining HCN2 in human hippocampal tissue by IHC-P (Bouin's fixed paraffin embedded).

Anti-HCN2 antibody [S71-37] (ab84817)参考文献

ab84817 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (HL-1 cardiac cell line)
Specification HL-1 cardiac cell line
Fixative Methanol
Permeabilization Yes - 0.1% Triton X-100 in PBS
Blocking step Goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
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提交于 Nov 04 2010

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