The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100. Detects a band of approximately 100,115 kDa.
Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Produces a large instantaneous current. Activated by cAMP. Modulated by intracellular chloride ions and pH; acidic pH shifts the activation to more negative voltages.
Highly expressed throughout the brain. Detected at low levels in heart.
Belongs to the potassium channel HCN family. Contains 1 cyclic nucleotide-binding domain.
The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
ab19346 has been successfully used in Western blot procedures. By Western blot, this antibody detects bands at ~100 kDa and ~115 kDa representing the non-glycosylated and glycosylated form of HCN2 in rat brain extract.
Kretschmannova K et al. The expression and role of hyperpolarization-activated and cyclic nucleotide-gated channels in endocrine anterior pituitary cells. Mol Endocrinol26:153-64 (2012).
Read more (PubMed: 22135067) »
Adams BE et al. Excitotoxic-mediated transcriptional decreases in HCN2 channel function increase network excitability in CA1. Exp Neurol219:249-57 (2009).
Read more (PubMed: 19500574) »