重组Anti-HCN1抗体[S70] (ab84816)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [S70] to HCN1
- Suitable for: IHC-P, IHC-Fr, WB, Flow Cyt
- Reacts with: Mouse, Rat, Human
概述
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产品名称
Anti-HCN1抗体[S70]
参阅全部 HCN1 一抗 -
描述
小鼠单克隆抗体[S70] to HCN1 -
宿主
Mouse -
特异性
No cross reactivity against HCN2
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经测试应用
适用于: IHC-P, IHC-Fr, WB, Flow Cytmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment within Rat HCN1 aa 750-950 (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q9JKB0 -
阳性对照
- WB: Rat brain lysate. IHC-P: Mouse cerebellum tissue, mouse brain tissue, human brain tissue. ICC: Human hippocampus tissue.
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常规说明
The clone number has been updated from S70-28 to N70/28, both clone numbers name the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
S70 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab84816于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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WB |
Use a concentration of 1 - 10 µg/ml. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa).
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Flow Cyt |
Use 1-2µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
说明 |
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IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
WB
Use a concentration of 1 - 10 µg/ml. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa). |
Flow Cyt
Use 1-2µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Activated by cAMP, and at 10-100 times higher concentrations, also by cGMP. May mediate responses to sour stimuli. -
组织特异性
Detected in brain, in particular in amygdala and hippocampus, while expression in caudate nucleus, corpus callosum, substantia nigra, subthalamic nucleus and thalamus is very low or not detectable. Detected at very low levels in muscle and pancreas. -
序列相似性
Belongs to the potassium channel HCN family.
Contains 1 cyclic nucleotide-binding domain. -
结构域
The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 348980 Human
- Entrez Gene: 15165 Mouse
- Entrez Gene: 84390 Rat
- Omim: 602780 Human
- SwissProt: O60741 Human
- SwissProt: O88704 Mouse
- SwissProt: Q9JKB0 Rat
- Unigene: 650434 Human
see all -
别名
- BCNG-1 antibody
- BCNG1 antibody
- Brain cyclic nucleotide gated channel 1 antibody
see all
图片
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Immunohistochemistry analysis of 10% formalin-fixed mouse cerebellum tissue labelling HCN1 with ab84816 at 1/1000 dilution for 1 hour at room temperature, followed by biotinylated Goat Anti-Mouse antibody (brown) for 30 minutes at room temperature. Localization: Cytoplasmic staining of Purkinje cells. Counterstain: Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at room temperature. HRP/DAB detection.
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Immunohistochemistry analysis of 10% formalin-fixed human frozen brain section labelling HCN1 with ab84816 at 1/1000 dilution for 1 hour at room temperature, followed by biotinylated Goat Anti-Mouse antibody (brown) for 30 minutes at room temperature. Localization: Neurons. Counterstain: Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at room temperature. HRP/DAB detection.
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Immunohistochemistry analysis of 10% formalin-fixed mouse frozen brain section labelling HCN1 with ab84816 at 1/1000 dilution for 1 hour at room temperature, followed by biotinylated Goat Anti-Mouse antibody (brown) for 30 minutes at room temperature. Counterstain: Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at room temperature. HRP/DAB detection.
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Overlay histogram showing SH-SY5Y cells stained with ab84816 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab84816, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween for 20 min used under the same conditions.
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Immunohistochemical analysis of paraffin-embedded (Bouin’s Fixative - picric acid, acetic acid and formaldehyde) human hippocampus tissue labelling HCN1 with ab84816 at 1/1000 dilution, for 1 hour at RT followed by secondary antibody Goat Anti-Mouse (FITC) at 1/50 dilution, for 1 hour at RT. Membrane and cytosolic staining on human hippocampus tissue is observed.
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Anti-HCN1 antibody [S70] (ab84816) at 1/1000 dilution + Rat brain membrane lysates at 15 µg
Secondary
HRP-Sheep Anti-Mouse IgG antibody
Predicted band size: 99 kDa
Observed band size: 99 kDa
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (16)
ab84816 被引用在 16 文献中.
- Lei X et al. Activation of HCN channels caused by elevated cAMP levels in periaqueductal gray promotes bone cancer pain. Neurochem Int 162:105437 (2023). PubMed: 36336089
- Lin W et al. Downregulation of hyperpolarization-activated cyclic nucleotide-gated channels (HCN) in the hippocampus of patients with medial temporal lobe epilepsy and hippocampal sclerosis (MTLE-HS). Hippocampus 30:1112-1126 (2020). PubMed: 32543742
- McClements ME et al. An AAV Dual Vector Strategy Ameliorates the Stargardt Phenotype in Adult Abca4-/- Mice. Hum Gene Ther N/A:N/A (2018). PubMed: 30381971
- Ding W et al. Increased HCN Channel Activity in the Gasserian Ganglion Contributes to Trigeminal Neuropathic Pain. J Pain 19:626-634 (2018). PubMed: 29366880
- He F et al. The role of HCN channels in peristaltic dysfunction in human ureteral tuberculosis. Int Urol Nephrol 50:639-645 (2018). WB, ICC/IF . PubMed: 29460132