概述

  • 产品名称
    Anti-HCN1抗体[N70/28]
    参阅全部 HCN1 一抗
  • 描述
    小鼠单克隆抗体[N70/28] to HCN1
  • 宿主
    Mouse
  • 特异性
    No cross reactivity against HCN2
  • 经测试应用
    适用于: IHC-P, IHC-Fr, IHC-FoFr, IP, WB, ICC/IF, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Fusion protein, corresponding to cytoplasmic C terminal amino acids 778-910 of Rat HCN1 (accession number Q9JKBo)

  • 阳性对照
    • Rat brain lysate
  • 常规说明

    The clone number has been updated from S70-28 to N70/28, both clone numbers name the same antibody clone.

     

性能

应用

Our Abpromise guarantee covers the use of ab84816 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration. PubMed: 22948144
IP Use at an assay dependent concentration.
WB Use a concentration of 1 - 10 µg/ml. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa).
ICC/IF Use a concentration of 1 - 10 µg/ml.
Flow Cyt Use 1-2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

靶标

  • 功能
    Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Activated by cAMP, and at 10-100 times higher concentrations, also by cGMP. May mediate responses to sour stimuli.
  • 组织特异性
    Detected in brain, in particular in amygdala and hippocampus, while expression in caudate nucleus, corpus callosum, substantia nigra, subthalamic nucleus and thalamus is very low or not detectable. Detected at very low levels in muscle and pancreas.
  • 序列相似性
    Belongs to the potassium channel HCN family.
    Contains 1 cyclic nucleotide-binding domain.
  • 结构域
    The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BCNG-1 antibody
    • BCNG1 antibody
    • Brain cyclic nucleotide gated channel 1 antibody
    • Brain cyclic nucleotide-gated channel 1 antibody
    • HAC 2 antibody
    • HAC2 antibody
    • HCN1 antibody
    • HCN1_HUMAN antibody
    • Hyperpolarization activated cyclic nucleotide gated potassium channel 1 antibody
    • Potassium channel, voltage-gated, brain, 1 antibody
    • Potassium/sodium hyperpolarization activated cyclic nucleotide gated channel 1 antibody
    • Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1 antibody
    see all

图片

  • Anti-HCN1 antibody [N70/28] (ab84816) + rat brain lysate

    Predicted band size: 99 kDa
    Observed band size: 99 kDa



    As secondary antibody a goat anti-mouse IgG conjugated with HRP was used.
  • 4% formalin-fixed human cerebellum tissue stained for HCN1 using ab84816 at 1/1000 dilution in immunohistochemical analysis. Counterstained with Hematoxylin. HRP/DAB detection.

  • 10% formalin-fixed frozen section of human brain tissue stained for HCN1 using ab84816 at 1/1000 dilution in immunohistochemical analysis. Counterstained with Hematoxylin. HRP/DAB detection.

  • 10% formalin-fixed frozen section of mouse brain tissue stained for HCN1 using ab84816 at 1/1000 dilution in immunohistochemical analysis. Counterstained with Hematoxylin. HRP/DAB detection.

  • Overlay histogram showing SH-SY5Y cells stained with ab84816 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab84816, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween for 20 min used under the same conditions.

  • ab84816 staining HCN1 in human hippocampal tissue by IHC-P (Bouin's fixed paraffin embedded).
  • Immunohistochemical analysis of murine cochlear tissue, staining HCN1 (red) with ab84816.

    Tissue was permeabilized with 0.3% Triton X-100 before being blocked with goat serum. Sections were incubated with primary antibody (1/100) overnight at 4°C. An AlexaFluor®568-conjugated goat anti-mouse IgG (1/2000) was used as the secondary antibody.

文献

This product has been referenced in:
  • He F  et al. The role of HCN channels in peristaltic dysfunction in human ureteral tuberculosis. Int Urol Nephrol 50:639-645 (2018). WB, ICC/IF . Read more (PubMed: 29460132) »
  • Dong X  et al. Interaction of Caveolin-3 and HCN is involved in the pathogenesis of diabetic cystopathy. Sci Rep 6:24844 (2016). IF . Read more (PubMed: 27122250) »

See all 8 Publications for this product

客户评价及客户问答

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (HL-1 cardiac cell line)
Specification
HL-1 cardiac cell line
Fixative
Methanol
Blocking step
Goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
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提交于 Nov 15 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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