HCLS1 overexpression 293T裂解液(全细胞) (ab94153)

概述

  • 产品名称HCLS1 overexpression 293T裂解液(全细胞)
  • 描述
    HCLS1 overexpression裂解液
  • 常规说明ab94153 is a 293T cell transfected lysate in which Human HCLS1 has been transiently over-expressed using a pCMV-HCLS1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • 经测试应用适用于: WBmore details

性能

  • 形式Liquid
  • 存放说明Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
  • 存储溶液pH: 6.80
    Constituent: 100% 1x Sample Buffer
  • Concentration information loading...
  • 纯度Whole Cell Lysate
  • 研究领域
  • 背景Developmental stage: Expressed in early stage of myeloid and erythroid differentiation. Function: Substrate of the antigen receptor-coupled tyrosine kinase. Plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells. May also be involved in the regulation of gene expression. PTM: Phosphorylated by LYN; rapidly after cross-linking of surface IgM on B-cells. Similarity: Contains 4 cortactin repeats. Contains 1 SH3 domain. Tissue specificity: Expressed only in tissues and cells of hematopoietic origin.

应用

Our Abpromise guarantee covers the use of ab94153 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution.

HCLS1 overexpression 293T lysate (whole cell) 图像

  • ab94153 at 15µg/lane on an SDS PAGE gel.
  • All lanes : Anti-HCLS1 antibody (ab55090) at 1/500 dilution

    Lane 1 : HCLS1 overexpression 293T lysate (whole cell) (ab94153)
    Lane 2 : 293T Non Transfected Lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

HCLS1 overexpression 293T lysate (whole cell) (ab94153)参考文献

ab94153 has not yet been referenced specifically in any publications.

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