Anti-HCLS1抗体(ab28725)

概述

  • 产品名称Anti-HCLS1抗体
    参阅全部 HCLS1 一抗
  • 描述
    兔多克隆抗体to HCLS1
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide: QRWGAKTIEG SGRTEHINIH QLRNKVSEEH DVLRKKEMES GPKASHGYGG, corresponding to N terminal amino acids 36-85 of Human HCLS1

  • 阳性对照
    • WB: Jurkat cell lysate. IHC-P: Human kidney tissue.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS.
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab28725 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 54 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能Substrate of the antigen receptor-coupled tyrosine kinase. Plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells. May also be involved in the regulation of gene expression.
  • 组织特异性Expressed only in tissues and cells of hematopoietic origin.
  • 序列相似性Contains 4 cortactin repeats.
    Contains 1 SH3 domain.
  • 发展阶段Expressed in early stage of myeloid and erythroid differentiation.
  • 翻译后修饰Phosphorylated by LYN; rapidly after cross-linking of surface IgM on B-cells.
  • 细胞定位Membrane. Cytoplasm. Mitochondrion.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cortactin like antibody
    • CTTNL antibody
    • HCLS 1 antibody
    • Hcls1 antibody
    • HCLS1_HUMAN antibody
    • Hematopoietic cell specific Lyn substrate 1 antibody
    • Hematopoietic cell-specific LYN substrate 1 antibody
    • Hematopoietic lineage cell-specific protein antibody
    • HS 1 antibody
    • HS1 antibody
    • LckBP1 antibody
    • OTTHUMP00000215180 antibody
    • OTTHUMP00000215182 antibody
    • p75 antibody
    see all

Anti-HCLS1 antibody 图像

  • Anti-HCLS1 antibody (ab28725) at 1.25 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti Rabbit IgG at 1/50000 dilution

    Predicted band size : 54 kDa
    Observed band size : 58 kDa (why is the actual band size different from the predicted?)
  • Ab28725, at a concentration of 4 - 8 µg/ml, staining HCLS1 in paraffin embedded human kidney tissue by Immunohistochemistry. Cells with positive label (as indicated by the arrows) are epithelial cells of the renal tubule. Magnification: 400X.
  • Immunohistochemistry of Human Heart lysate tissue at an antibody concentration of 4-8µg/ml using anti-HCLS1 antibody (ab28725)

  • Immunohistochemistry of Human Stomach tissue at an antibody concentration of 4-8µg/ml using anti-HCLS1 antibody (ab28725)

Anti-HCLS1 antibody (ab28725)参考文献

ab28725 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. The bands in molecular weight marker we used before are a little deviate from the expected locations, therefore the 60kD band is about 70kD according to our new calibrated marker. This product should detect a band at arou...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"