Anti-Hck (phospho Y410)抗体(ab61055)

概述

  • 产品名称
    Anti-Hck (phospho Y410)抗体
    参阅全部 Hck 一抗
  • 描述
    兔多克隆抗体to Hck (phospho Y410)
  • 特异性
    Detects target only when phosphorylated at tyrosine 410.
  • 经测试应用
    适用于: IHC-P, ICC/IF, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthetic phosphopeptide derived from human Hck around the phosphorylation site of tyrosine 410 (N-E-YP-T-A)

  • 阳性对照
    • HeLa cells

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab61055 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
ICC/IF 1/500 - 1/1000.
ELISA 1/20000.

靶标

  • 功能
    May serve as part of a signaling pathway coupling the Fc receptor to the activation of the respiratory burst. May also contribute to neutrophil migration and may regulate the degranulation process of neutrophils.
  • 组织特异性
    Expressed predominantly in cells of the myeloid and B-lymphoid lineages.
  • 序列相似性
    Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • 结构域
    The SH3 domain mediates binding to HIV-1 Nef.
  • 细胞定位
    Membrane. Cytoplasm. At least half of this isoform is found in the cytoplasm, some of this fraction is myristoylated and Membrane. Membrane > caveola. 20% of this isoform is associated with caveolae, this association depends on palmitoylation of cyteine at position 3.
  • Information by UniProt
  • 数据库链接
  • 形式
    This protein is known to be similar in amino acid sequence to LCK (P06239), FYN (P06241), YES1 (P07947), SRC (P12931), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request.
  • 别名
    • Bmk antibody
    • Hck 1 antibody
    • Hck antibody
    • HCK_HUMAN antibody
    • Hemopoietic cell kinase antibody
    • JTK9 antibody
    • p59-HCK/p60-HCK antibody
    • p59Hck antibody
    • p59HCK/p60HCK antibody
    • p61Hck antibody
    • Tyrosine protein kinase HCK antibody
    • Tyrosine-protein kinase HCK antibody
    see all

图片

  • ab61055 staining Hck (phospho Y410) in Mouse lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 10% formalin and blocked with 10% BSA for 1 hour; antigen retrieval was by heat mediation in trypsin. Samples were incubated with primary antibody (1/100) for 24 hours. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • 1/500-1/1000 ab61055 staining phosphorilated Hck on HeLa cells in the absence (left) or presence (right) of the immunizing phosphopeptide.

文献

ab61055 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Trypsin
Sample
Mouse Tissue sections (Lung)
Specification
Lung
Permeabilization
No
Fixative
10% Formalin
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提交于 Oct 30 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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