RabMAb

Anti-Hck抗体[EP2415Y] (ab75839)

概述

  • 产品名称Anti-Hck抗体[EP2415Y]
    参阅全部 Hck 一抗
  • 描述
    兔单克隆抗体[EP2415Y] to Hck
  • 特异性This protein is known to be similar in amino acid sequence to LCK (P06239), FYN (P06241), YES1 (P07947), SRC (P12931), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request, please contact our Scientific Support for further information.
  • 经测试应用适用于: WB, IP, ICC, Flow Cytmore details
    不适用于: IHC-P
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic peptide corresponding to residues near the C terminus of human Hck

  • 阳性对照
    • TF-1 cell lysate.
  • 常规说明

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab75839 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/20000 - 1/200000. Predicted molecular weight: 59 kDa.
IP 1/30.
ICC 1/100 - 1/250.
Flow Cyt 1/60.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • 应用说明Is unsuitable for IHC-P.
  • 靶标

    • 功能May serve as part of a signaling pathway coupling the Fc receptor to the activation of the respiratory burst. May also contribute to neutrophil migration and may regulate the degranulation process of neutrophils.
    • 组织特异性Expressed predominantly in cells of the myeloid and B-lymphoid lineages.
    • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
      Contains 1 protein kinase domain.
      Contains 1 SH2 domain.
      Contains 1 SH3 domain.
    • 结构域The SH3 domain mediates binding to HIV-1 Nef.
    • 细胞定位Membrane. Cytoplasm. At least half of this isoform is found in the cytoplasm, some of this fraction is myristoylated and Membrane. Membrane > caveola. 20% of this isoform is associated with caveolae, this association depends on palmitoylation of cyteine at position 3.
    • Information by UniProt
    • 数据库链接
    • 形式This protein is known to be similar in amino acid sequence to LCK (P06239), FYN (P06241), YES1 (P07947), SRC (P12931), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request.
    • 别名
      • Bmk antibody
      • Hck 1 antibody
      • Hck antibody
      • HCK_HUMAN antibody
      • Hemopoietic cell kinase antibody
      • JTK9 antibody
      • p59-HCK/p60-HCK antibody
      • p59Hck antibody
      • p59HCK/p60HCK antibody
      • p61Hck antibody
      • Tyrosine protein kinase HCK antibody
      • Tyrosine-protein kinase HCK antibody
      see all

    Anti-Hck antibody [EP2415Y] 图像

    • Anti-Hck antibody [EP2415Y] (ab75839) at 1/200000 dilution + TF-1 cell lysate at 10 µg

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 59 kDa
      Observed band size : 59 kDa

    Anti-Hck antibody [EP2415Y] (ab75839)参考文献

    ab75839 has not yet been referenced specifically in any publications.

    Product Wall

    We have not tested ab75839 in-house for cross reactivity with LCK,YES, or BLK so we cannot say for certain whether this antibody would be suitable for detecting these targets. However, in our WB tests we have only observed a single band at ~ 60 KDa wh...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"