The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).
Use at an assay dependent dilution. Reported to Immunoprecipitate Hck from 1-2 x 10 7 THP-1 monocytic cells lysed in RIPA.
功能May serve as part of a signaling pathway coupling the Fc receptor to the activation of the respiratory burst. May also contribute to neutrophil migration and may regulate the degranulation process of neutrophils.
组织特异性Expressed predominantly in cells of the myeloid and B-lymphoid lineages.
序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. Contains 1 protein kinase domain. Contains 1 SH2 domain. Contains 1 SH3 domain.
结构域The SH3 domain mediates binding to HIV-1 Nef.
细胞定位Membrane. Cytoplasm. At least half of this isoform is found in the cytoplasm, some of this fraction is myristoylated and Membrane. Membrane > caveola. 20% of this isoform is associated with caveolae, this association depends on palmitoylation of cyteine at position 3.
形式This protein is known to be similar in amino acid sequence to LCK (P06239), FYN (P06241), YES1 (P07947), SRC (P12931), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request.
Hck 1 antibody
Hemopoietic cell kinase antibody
Tyrosine protein kinase HCK antibody
Tyrosine-protein kinase HCK antibody
Anti-Hck antibody (ab32860)参考文献
This product has been referenced in:
Xiao X et al. c-Yes regulates cell adhesion at the apical ectoplasmic specialization-blood-testis barrier axis via its effects on protein recruitment and distribution. Am J Physiol Endocrinol Metab304:E145-59 (2013).
Read more (PubMed: 23169788) »