Anti-hCG beta抗体[HCG-61] (ab764)

概述

  • 产品名称Anti-hCG beta抗体[HCG-61]
    参阅全部 hCG beta 一抗
  • 描述
    小鼠单克隆抗体[HCG-61] to hCG beta
  • 特异性Reacts with beta subunit of human chorionic gonadotropin with association constant 5.1 x 1010 l/mol. The following cross-reactivities (%) were determined by classic double-antibody radioimmunoassay using unlabelled hormones: b-hCG = 77 a-hCG = 1.3 hLH = 0.86 hFSH = < 0.5 hTSH = < 0.5
  • 经测试应用适用于: ELISA, ICC, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length native protein (purified) (Human).

  • 阳性对照
    • This antibody gave a positive result in IHC in the following FFPE tissue: Human normal placenta.

性能

应用

Our Abpromise guarantee covers the use of ab764 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use a concentration of 3 - 10 µg/ml.
ICC Use a concentration of 3 - 10 µg/ml.
IHC-P Use a concentration of 5 µg/ml.

靶标

Anti-hCG beta antibody [HCG-61] 图像

  • IHC image of hCG beta staining in Human normal placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab764, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-hCG beta antibody [HCG-61] (ab764)参考文献

ab764 has not yet been referenced specifically in any publications.

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