Anti-HCF-1 / Host Cell Factor C1抗体(ab110896)

概述

  • 产品名称Anti-HCF-1 / Host Cell Factor C1抗体
    参阅全部 HCF-1 / Host Cell Factor C1 一抗
  • 描述
    兔多克隆抗体to HCF-1 / Host Cell Factor C1
  • 经测试应用适用于: IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide derived from an internal sequence at N-terminal of Human HCF-1/ Host Cell Factor C1.

  • 阳性对照
    • Human breast carcinoma tissue

性能

应用

Our Abpromise guarantee covers the use of ab110896 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Involved in control of the cell cycle. Also antagonizes transactivation by ZBTB17 and GABP2; represses ZBTB17 activation of the p15(INK4b) promoter and inhibits its ability to recruit p300. Coactivator for EGR2 and GABP2. Tethers the chromatin modifying Set1/Ash2 histone H3 'Lys-4' methyltransferase (H3K4me) and Sin3 histone deacetylase (HDAC) complexes (involved in the activation and repression of transcription, respectively) together. In case of human herpes simplex virus (HSV) infection, HCFC1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and POU2F1 thereby enabling the transcription of the viral immediate early genes.
  • 组织特异性Highly expressed in fetal tissues and the adult kidney. Present in all tissues tested.
  • 序列相似性Contains 5 Kelch repeats.
  • 结构域The HCF repeat is a highly specific proteolytic cleavage signal.
    The kelch repeats fold into a 6-bladed kelch beta-propeller called the beta-propeller domain which mediates interaction with HCFC1R1.
  • 翻译后修饰Proteolytically cleaved at one or several PPCE--THET sites within the HCF repeats. Further cleavage of the primary N- and C-terminal chains results in a 'trimming' and accumulation of the smaller chains.
    O-glycosylated.
    Ubiquitinated. Lys-1807 and Lys-1808 are ubiquitinated both via 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. BAP1 mediated deubiquitination of 'Lys-48'-linked polyubiquitin chains; deubiquitination by BAP1 does not seem to stabilize the protein.
  • 细胞定位Cytoplasm. Nucleus. HCFC1R1 modulates its subcellular localization and overexpression of HCFC1R1 leads to accumulation of HCFC1 in the cytoplasm. Nuclear in general, but uniquely cytoplasmic in trigeminal ganglia, becoming nuclear upon HSV reactivation from the latent state.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C1 factor antibody
    • CFF antibody
    • HCF 1 antibody
    • HCF antibody
    • HCF C-terminal chain 6 antibody
    • HCF-1 antibody
    • HCF1 antibody
    • Hcfc1 antibody
    • HCFC1_HUMAN antibody
    • HFC1 antibody
    • Host cell factor 1 antibody
    • Host cell factor antibody
    • Host cell factor C1 (VP16 accessory protein) antibody
    • Host cell factor C1 antibody
    • MGC70925 antibody
    • MRX3 antibody
    • VCAF antibody
    • VP16 accessory protein antibody
    see all

Anti-HCF-1 / Host Cell Factor C1 antibody 图像

  • ab110896 at 1/50 dilution staining HCF-1 / Host Cell Factor C1 in paraffin-embedded Human Breast carcinoma tissue by Immunohistochemistry. The image on the right is treated with the synthesized peptide.

Anti-HCF-1 / Host Cell Factor C1 antibody (ab110896)参考文献

This product has been referenced in:
  • Zhou P  et al. Mixed lineage leukemia 5 (MLL5) protein regulates cell cycle progression and E2F1-responsive gene expression via association with host cell factor-1 (HCF-1). J Biol Chem 288:17532-43 (2013). Read more (PubMed: 23629655) »

See 1 Publication for this product

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