概述

  • 产品名称Anti-HCE抗体
    参阅全部 HCE 一抗
  • 描述
    羊多克隆抗体to HCE
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein (Human) with 6x His tag

性能

应用

Our Abpromise guarantee covers the use of ab27971 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
IP Use at an assay dependent dilution.

靶标

  • 功能Bifunctional mRNA-capping enzyme exhibiting RNA 5'-triphosphatase activity in the N-terminal part and mRNA guanylyltransferase activity in the C-terminal part. Catalyzes the first two steps of cap formation: by removing the gamma-phosphate from the 5'-triphosphate end of nascent mRNA to yield a diphosphate end, and by transferring the gmp moiety of GTP to the 5'-diphosphate terminus.
  • 组织特异性Isoform 1 and isoform 4 (at a lesser extent) are expressed in cerebrum, cerebellum, thyroid, lung, heart, liver, kidney, spleen, large intestine, testis, skin and muscle.
  • 序列相似性In the N-terminal section; belongs to the non-receptor class of the protein-tyrosine phosphatase family.
    In the C-terminal section; belongs to the eukaryotic GTase family.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CAP1A antibody
    • GTase antibody
    • GTP--RNA guanylyltransferase antibody
    • hCAP antibody
    • HCAP1 antibody
    • HcE antibody
    • HCE1 antibody
    • MCE1 antibody
    • MCE1_HUMAN antibody
    • mRNA 5''-triphosphatase antibody
    • mRNA capping enzyme antibody
    • mRNA guanyltransferase antibody
    • mRNA guanylyltransferase antibody
    • Polynucleotide 5' trisphosphatase antibody
    • RNA guanylyltransferase and 5' phosphatase antibody
    • RNGTT antibody
    • TPase antibody
    see all

Anti-HCE antibody 图像

  • All lanes : Anti-HCE antibody (ab27971) at 1/1000 dilution

    Lane 1 : Hela nuclear extract at 0.5 µl
    Lane 2 : Hela nuclear extract at 1 µl

    Secondary
    donkey anti-sheep peroxidase conjugate at 1/20000 dilution

    Predicted band size : 68 kDa
    Observed band size : 68 kDa

Anti-HCE antibody (ab27971)参考文献

This product has been referenced in:
  • Adamson TE  et al. Functional coupling of cleavage and polyadenylation with transcription of mRNA. J Biol Chem 280:32262-71 (2005). Read more (PubMed: 16041059) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"