概述

  • 产品名称
  • 描述
    山羊多克隆抗体to HCCA2
  • 特异性
    ab115013 is expected to recognize both reported isoforms (NP_001165694.1; NP_443731.2) only.
  • 经测试应用
    适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Dog
  • 免疫原

    Synthetic peptide:

    DERGKKVKCTAPQ

    , corresponding to internal sequence amino acids 128-140 of Human HCCA2 (NP_001165694.1; NP_443731.2).

  • 阳性对照
    • Jurkat cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab115013 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.02 - 0.06 µg/ml. Detects a band of approximately 30, 48 kDa (predicted molecular weight: 30 kDa). An additional band of unknown identity was also consistently observed at 48kDa. This band was successfully blocked by incubation with the immunizing peptide.

靶标

图片

  • ab115013 (5µg/ml) shows staining of HCCA2 in paraffin embedded Human Lung following steamed antigen retrieval with citrate buffer pH 6 and AP-staining. This shows staining of smooth muscle cells, vasculature and apical secretory granules in the epithelium of the bronchiole.

  • Anti-HCCA2 antibody (ab115013) at 0.02 µg/ml + Jurkat cell lysate at 35 µg
    Developed using the ECL technique

    Predicted band size : 30 kDa

文献

ab115013 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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