Anti-HCCA2抗体(ab115013)
Key features and details
- Goat polyclonal to HCCA2
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-HCCA2抗体 -
描述
山羊多克隆抗体to HCCA2 -
宿主
Goat -
特异性
ab115013 is expected to recognize both reported isoforms (NP_001165694.1; NP_443731.2) only. -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow, Dog -
免疫原
Synthetic peptide:
DERGKKVKCTAPQ
, corresponding to internal sequence amino acids 128-140 of Human HCCA2 (NP_001165694.1; NP_443731.2). -
阳性对照
- Jurkat cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab115013 was purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab115013于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.02 - 0.06 µg/ml. Detects a band of approximately 30, 48 kDa (predicted molecular weight: 30 kDa).
A 1 hour primary incubation is recommended for this product. An additional band of unknown identity was also consistently observed at 48kDa. This band was successfully blocked by incubation with the immunizing peptide. |
说明 |
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IHC-P
Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.02 - 0.06 µg/ml. Detects a band of approximately 30, 48 kDa (predicted molecular weight: 30 kDa). A 1 hour primary incubation is recommended for this product. An additional band of unknown identity was also consistently observed at 48kDa. This band was successfully blocked by incubation with the immunizing peptide. |
靶标
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功能
Stimulates the autophosphorylation and kinase activity of STK38 and STK38L. -
序列相似性
Belongs to the MOB1/phocein family. -
翻译后修饰
Phosphorylated. -
细胞定位
Nucleus. Cytoplasm > perinuclear region. - Information by UniProt
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数据库链接
- Entrez Gene: 81532 Human
- Entrez Gene: 101513 Mouse
- Entrez Gene: 499288 Rat
- Omim: 611969 Human
- SwissProt: Q70IA6 Human
- SwissProt: Q8VI63 Mouse
- Unigene: 370360 Human
- Unigene: 53259 Mouse
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别名
- HCCA2 antibody
- HCCA2 gene antibody
- HCCA2 protein antibody
see all
图片
数据表及文件
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Datasheet download
文献 (0)
ab115013 尚未被引用在任何文献中。