Anti-HCC1抗体(ab12326)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to HCC1
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to Human HCC1 aa 480-530.
    (Peptide available as ab40426)

  • 阳性对照
    • Nuclear extract from HeLa cells.
  • 常规说明
    Centrifuge tube to remove product from lid.

性能

应用

Our Abpromise guarantee covers the use of ab12326 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 59 kDa.

靶标

  • 功能
    Transcriptional coactivator for steroid nuclear receptors ESR1/ER-alpha and ESR2/ER-beta, and JUN/AP-1 (By similarity). May be involved in pre-mRNA splicing process.
  • 组织特异性
    Widely expressed. Highly expressed in pancreas, skeletal muscle, lung and brain. Expressed at intermediate level in kidney, liver and heart.
  • 序列相似性
    Belongs to the splicing factor SR family.
    Contains 3 RRM (RNA recognition motif) domains.
  • 细胞定位
    Nucleus speckle. Concentrated in nuclear speckles. Colocalizes with the core spliceosomal snRNP proteins.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CAPER alpha antibody
    • CAPER antibody
    • CAPERalpha antibody
    • CC1.3 antibody
    • Coactivator of activating protein 1 and estrogen receptors antibody
    • Coactivator of AP1 and ERs antibody
    • DKFZp781C0423 antibody
    • FLJ44170 antibody
    • HCC 1 antibody
    • Hepatocellular carcinoma protein 1 antibody
    • RBM 39 antibody
    • RBM39 antibody
    • RBM39_HUMAN antibody
    • RNA binding motif protein 39 antibody
    • RNA binding region (RNP1 RRM) containing 2 antibody
    • RNA binding region containing 2 antibody
    • RNA binding region containing protein 2 antibody
    • RNA-binding motif protein 39 antibody
    • RNA-binding protein 39 antibody
    • RNA-binding region-containing protein 2 antibody
    • RNPC 2 antibody
    • RNPC2 antibody
    • Splicing factor CC1.3 antibody
    • Splicing factor CC1.4 antibody
    • Splicing factor HCC 1 antibody
    • Splicing factor HCC1 antibody
    • Transcription coactivator CAPER antibody
    see all

图片



  • Predicted band size : 59 kDa


    Detection of Human Caper by Western Blot. Samples: Nuclear extract (NE) from HeLa cells. Antibody: ab12326 used at 0.3 mcg/ml. Detection: Chemiluminescence with an exposure time of 5 minutes.

实验方案

文献

ab12326 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa, 293, U2OS)
Loading amount
30 µg
Specification
HeLa, 293, U2OS
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

提交于 Jan 29 2007

Thank you for your enquiry. I am sorry for the confusion, HCC1 antibody (ab12326) detects a protein with a predicted molecular weight of 58KDa. However, the actual band as detailed on the western image is more like 70KDa. The observed molecular ...

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Thank you for your enquiry. First, I'm pleased to hear that ab12326 is working out for you in Wetern blotting. Ab12326 has not yet been tested for application in IP. I do suggest that you submit a review regarding your experiences with the antibody - i...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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