概述

  • 产品名称Anti-hCAP-D3抗体
    参阅全部 hCAP-D3 一抗
  • 描述
    兔多克隆抗体to hCAP-D3
  • 特异性We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Rhesus monkey, Gorilla
  • 免疫原

    Synthetic peptide corresponding to a region between C terminal residues 1450-1507 of human hCAP-D3. (NP_056076.1)

  • 阳性对照
    • HeLa and 293T whole cell lysate

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab70349 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
  • 应用说明IP: Use at 1 to 4 µg/mg of lysate
    WB: 1/2000 - 1/10000. Detects a band of approximately 174 kDa (predicted molecular weight: 168 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Regulatory subunit of the condensin-2 complex, a complex which establishes mitotic chromosome architecture and is involved in physical rigidity of the chromatid axis.
    • 序列相似性Contains 4 HEAT repeats.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CAP D3 antibody
      • CAPD 3 antibody
      • CAPD3 antibody
      • CNDD3_HUMAN antibody
      • Condensin II complex subunit D3 antibody
      • Condensin-2 complex subunit D3 antibody
      • FLJ42888 antibody
      • hCAP-D3 antibody
      • Hcp 6 antibody
      • Hcp6 antibody
      • hHCP 6 antibody
      • hHCP6 antibody
      • KIAA0056 antibody
      • MGC104671 antibody
      • NCAPD 3 antibody
      • Ncapd3 antibody
      • Non SMC condensin II complex subunit D3 antibody
      • Non-SMC condensin II complex subunit D3 antibody
      • OTTHUMP00000235403 antibody
      • OTTHUMP00000235404 antibody
      see all

    Anti-hCAP-D3 antibody 图像

    • All lanes:200µg Hela whole cell lysate and ab70349 at 1µg/ml. Lane 1: ab70349 at 3 µg/mg lysate for IP. Lane 2: IgG control.
    • All lanes : Anti-hCAP-D3 antibody (ab70349) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg


      Predicted band size : 168 kDa
      Observed band size : 174 kDa (why is the actual band size different from the predicted?)

    Anti-hCAP-D3 antibody (ab70349)参考文献

    This product has been referenced in:
    • Liu W  et al. PHF8 mediates histone H4 lysine 20 demethylation events involved in cell cycle progression. Nature 466:508-12 (2010). WB ; Human . Read more (PubMed: 20622854) »
    • Centore RC  et al. CRL4(Cdt2)-mediated destruction of the histone methyltransferase Set8 prevents premature chromatin compaction in S phase. Mol Cell 40:22-33 (2010). WB ; Human . Read more (PubMed: 20932472) »

    See all 2 Publications for this product

    Product Wall

    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application Western blot
    Sample Mouse Cell lysate - whole cell (fibroblast)
    Loading amount 50 µg
    Specification fibroblast
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

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    提交于 May 10 2013

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