概述

  • 产品名称
    Anti-hCAP-D3抗体
    参阅全部 hCAP-D3 一抗
  • 描述
    兔多克隆抗体to hCAP-D3
  • 特异性
    We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • 经测试应用
    适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Rhesus monkey, Gorilla
  • 免疫原

    Synthetic peptide corresponding to a region between C terminal residues 1450-1507 of human hCAP-D3. (NP_056076.1)

  • 阳性对照
    • HeLa and 293T whole cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab70349 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
  • 应用说明
    IP: Use at 1 to 4 µg/mg of lysate
    WB: 1/2000 - 1/10000. Detects a band of approximately 174 kDa (predicted molecular weight: 168 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Regulatory subunit of the condensin-2 complex, a complex which establishes mitotic chromosome architecture and is involved in physical rigidity of the chromatid axis.
    • 序列相似性
      Contains 4 HEAT repeats.
    • 细胞定位
      Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CAP D3 antibody
      • CAPD 3 antibody
      • CAPD3 antibody
      • CNDD3_HUMAN antibody
      • Condensin II complex subunit D3 antibody
      • Condensin-2 complex subunit D3 antibody
      • FLJ42888 antibody
      • hCAP-D3 antibody
      • Hcp 6 antibody
      • Hcp6 antibody
      • hHCP 6 antibody
      • hHCP6 antibody
      • KIAA0056 antibody
      • MGC104671 antibody
      • NCAPD 3 antibody
      • Ncapd3 antibody
      • Non SMC condensin II complex subunit D3 antibody
      • Non-SMC condensin II complex subunit D3 antibody
      • OTTHUMP00000235403 antibody
      • OTTHUMP00000235404 antibody
      see all

    图片

    • All lanes:200µg Hela whole cell lysate and ab70349 at 1µg/ml. Lane 1: ab70349 at 3 µg/mg lysate for IP. Lane 2: IgG control.
    • All lanes : Anti-hCAP-D3 antibody (ab70349) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg


      Predicted band size : 168 kDa
      Observed band size : 174 kDa (why is the actual band size different from the predicted?)

    文献

    This product has been referenced in:

    See all 3 Publications for this product

    客户评价及客户问答

    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (fibroblast)
    Loading amount
    50 µg
    Specification
    fibroblast
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 May 10 2013

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