重组
RabMAb

Anti-HB EGF抗体[EPR13276(2)] (ab185555)

概述

  • 产品名称
    Anti-HB EGF抗体[EPR13276(2)]
    参阅全部 HB EGF 一抗
  • 描述
    兔单克隆抗体[EPR13276(2)] to HB EGF
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Synthetic peptide within Human HB EGF aa 50-150. The exact sequence is proprietary.
    Database link: Q99075

  • 阳性对照
    • HeLa and K563 cell lysates.
  • 常规说明

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab185555 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/50000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).

靶标

  • 功能
    May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts and smooth muscle but not endothelial cells. It is able to bind EGF receptors with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.
  • 序列相似性
    Contains 1 EGF-like domain.
  • 翻译后修饰
    Several N-termini have been identified by direct sequencing. The forms with N-termini 63, 73 and 74 have been tested and found to be biologically active.
    O-linked glycan attachment sites were determined by Edman degradation, O-glycanase digest suggests mucin-type glycosylation (done in HB-EGF purified from histiocytic lymphoma cell line U-937).
  • 细胞定位
    Cell membrane and Secreted > extracellular space. Mature HB-EGF is released into the extracellular space and probably binds to a receptor.
  • Information by UniProt
  • 数据库链接
  • 别名
    • diphtheria toxin receptor (heparin-binding EGF-like growth factor) antibody
    • diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor) antibody
    • Diphtheria toxin receptor antibody
    • DT R antibody
    • DT-R antibody
    • DTR antibody
    • DTS antibody
    • DTSF antibody
    • HB-EGF antibody
    • HBEGF antibody
    • HBEGF_HUMAN antibody
    • HEGFL antibody
    • Heparin binding EGF like growth factor antibody
    • Heparin binding epidermal growth factor antibody
    • Heparin binding epidermal growth factor like growth factor antibody
    • Heparin-binding EGF-like growth factor antibody
    • Proheparin binding EGF like growth factor antibody
    see all

图片

  • Western blot analysis on immunoprecipitation from 1) K562 cell lysate and 2) PBS, labeling HB EGF using ab185555 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.

  • All lanes : Anti-HB EGF antibody [EPR13276(2)] (ab185555) at 1/20000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : K562 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 23 kDa
    Observed band size: 23 kDa

实验方案

文献

ab185555 has not yet been referenced specifically in any publications.

客户评价及客户问答

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Vascular smooth muscle cells)
Gel Running Conditions
Reduced Denaturing (15)
Loading amount
30 µg
Treatment
agonist 1 & 2 for 24 hrs
Specification
Vascular smooth muscle cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Mr. Mc Shen

Verified customer

提交于 Feb 11 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Heart)
Gel Running Conditions
Reduced Denaturing (15)
Loading amount
60 µg
Treatment
MI
Specification
Heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. 474 HMRC

Verified customer

提交于 Feb 09 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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