Anti-HB EGF抗体[4G10] (Biotin) (ab66793)

概述

  • 产品名称
    Anti-HB EGF抗体[4G10] (Biotin)
    参阅全部 HB EGF 一抗
  • 描述
    小鼠单克隆抗体[4G10] to HB EGF (Biotin)
  • 偶联物
    Biotin
  • 经测试应用
    适用于: IP, ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    不与反应: Mouse
  • 免疫原

    Recombinant human HB EGF ectodomain expressed in SF21 cells.

  • 表位
    EGF domain
  • 阳性对照
    • Vero cell extract carrying human HG-EGF expression vector.
  • 常规说明

    Biotin/IgG = 7.5

性能

应用

Our Abpromise guarantee covers the use of ab66793 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.
WB Use a concentration of 0.2 - 1 µg/ml. Detects a band of approximately 20-28 kDa (predicted molecular weight: 23 kDa).

靶标

  • 功能
    May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts and smooth muscle but not endothelial cells. It is able to bind EGF receptors with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.
  • 序列相似性
    Contains 1 EGF-like domain.
  • 翻译后修饰
    Several N-termini have been identified by direct sequencing. The forms with N-termini 63, 73 and 74 have been tested and found to be biologically active.
    O-linked glycan attachment sites were determined by Edman degradation, O-glycanase digest suggests mucin-type glycosylation (done in HB-EGF purified from histiocytic lymphoma cell line U-937).
  • 细胞定位
    Cell membrane and Secreted > extracellular space. Mature HB-EGF is released into the extracellular space and probably binds to a receptor.
  • Information by UniProt
  • 数据库链接
  • 别名
    • diphtheria toxin receptor (heparin-binding EGF-like growth factor) antibody
    • diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor) antibody
    • Diphtheria toxin receptor antibody
    • DT R antibody
    • DT-R antibody
    • DTR antibody
    • DTS antibody
    • DTSF antibody
    • HB-EGF antibody
    • HBEGF antibody
    • HBEGF_HUMAN antibody
    • HEGFL antibody
    • Heparin binding EGF like growth factor antibody
    • Heparin binding epidermal growth factor antibody
    • Heparin binding epidermal growth factor like growth factor antibody
    • Heparin-binding EGF-like growth factor antibody
    • Proheparin binding EGF like growth factor antibody
    see all

图片

  • These images shows Vero cells carrying human HG-EGF expression vector (left hand image) and Vero cells carrying mouse HB-EGF expression vector (right hand image). Cells treated with ab66793, fixed with 4% PFA and reacted with Cys3 conjugated 2nd antibody.
  • All lanes : HB EGF antibody [4G10](biotin) - Azide free (ab66793) at 1 µg/ml. Lane 1 : Vero cell extract Lane 2 : Vero cell extract from cells carrrying human HB-EGF expression vector. Lane 3 : Vero cell extract from cells carrying mouse HB-EGF expression vector Predicted band size : 23 kDa Observed band size : 20-38 kDa Additional bands at : 41 kDa. We are unsure as to the identity of these extra bands. This image shows Immunoprecipitation of HB EGF followed by Western blotting/SDS PAGE; the cell surface was biotinylated.
  • All lanes : Anti-HB EGF antibody [4G10] (Biotin) (ab66793) at 1 µg/ml

    Lane 1 : Vero cell extract
    Lane 2 : Vero cell extract from cells carrrying human HB-EGF expression vector.
    Lane 3 : Vero cell extract from cells carrying mouse HB-EGF expression vector.


    Predicted band size : 23 kDa
    Observed band size : 20-28 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 41 kDa. We are unsure as to the identity of these extra bands.

文献

ab66793 has not yet been referenced specifically in any publications.

客户评价及客户问答

Thank you for your inquiry. You probably could use an anti-mouse secondary antibody with ab66793 since we know it worked well with a Cy3-conjugated secondary antibody. But, there are 7.5 biotin molecules per IgG1 molecule, and that could cause ...

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We could not find the sequence of Macaque monkey HB-EGF in databases. However, since the green monkey HB-EGF sequence has 97% identity to that of human, there is a good chance that ab66793 react with Macaque HB-EGF. Though the human HB-EGF has 80% iden...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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