Anti-HB EGF抗体[4G10] (Biotin) (ab66793)

概述

  • 产品名称Anti-HB EGF抗体[4G10] (Biotin)
    参阅全部 HB EGF 一抗
  • 描述
    小鼠单克隆抗体[4G10] to HB EGF (Biotin)
  • 偶联物Biotin
  • 经测试应用适用于: IP, ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    不与反应: Mouse
  • 免疫原

    Recombinant human HB EGF ectodomain expressed in SF21 cells.

  • 表位EGF domain
  • 阳性对照
    • Vero cell extract carrying human HG-EGF expression vector.
  • 常规说明

    Biotin/IgG = 7.5

性能

应用

Our Abpromise guarantee covers the use of ab66793 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.
WB Use a concentration of 0.2 - 1 µg/ml. Detects a band of approximately 20-28 kDa (predicted molecular weight: 23 kDa).

靶标

  • 功能May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts and smooth muscle but not endothelial cells. It is able to bind EGF receptors with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.
  • 序列相似性Contains 1 EGF-like domain.
  • 翻译后修饰Several N-termini have been identified by direct sequencing. The forms with N-termini 63, 73 and 74 have been tested and found to be biologically active.
    O-linked glycan attachment sites were determined by Edman degradation, O-glycanase digest suggests mucin-type glycosylation (done in HB-EGF purified from histiocytic lymphoma cell line U-937).
  • 细胞定位Cell membrane and Secreted > extracellular space. Mature HB-EGF is released into the extracellular space and probably binds to a receptor.
  • Information by UniProt
  • 数据库链接
  • 别名
    • diphtheria toxin receptor (heparin-binding EGF-like growth factor) antibody
    • diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor) antibody
    • Diphtheria toxin receptor antibody
    • DT R antibody
    • DT-R antibody
    • DTR antibody
    • DTS antibody
    • DTSF antibody
    • HB-EGF antibody
    • HBEGF antibody
    • HBEGF_HUMAN antibody
    • HEGFL antibody
    • Heparin binding EGF like growth factor antibody
    • Heparin binding epidermal growth factor antibody
    • Heparin binding epidermal growth factor like growth factor antibody
    • Heparin-binding EGF-like growth factor antibody
    • Proheparin binding EGF like growth factor antibody
    see all

Anti-HB EGF antibody [4G10] (Biotin) 图像

  • These images shows Vero cells carrying human HG-EGF expression vector (left hand image) and Vero cells carrying mouse HB-EGF expression vector (right hand image). Cells treated with ab66793, fixed with 4% PFA and reacted with Cys3 conjugated 2nd antibody.
  • All lanes : HB EGF antibody [4G10](biotin) - Azide free (ab66793) at 1 µg/ml. Lane 1 : Vero cell extract Lane 2 : Vero cell extract from cells carrrying human HB-EGF expression vector. Lane 3 : Vero cell extract from cells carrying mouse HB-EGF expression vector Predicted band size : 23 kDa Observed band size : 20-38 kDa Additional bands at : 41 kDa. We are unsure as to the identity of these extra bands. This image shows Immunoprecipitation of HB EGF followed by Western blotting/SDS PAGE; the cell surface was biotinylated.
  • All lanes : Anti-HB EGF antibody [4G10] (Biotin) (ab66793) at 1 µg/ml

    Lane 1 : Vero cell extract
    Lane 2 : Vero cell extract from cells carrrying human HB-EGF expression vector.
    Lane 3 : Vero cell extract from cells carrying mouse HB-EGF expression vector.


    Predicted band size : 23 kDa
    Observed band size : 20-28 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 41 kDa. We are unsure as to the identity of these extra bands.

Anti-HB EGF antibody [4G10] (Biotin) (ab66793)参考文献

ab66793 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your inquiry. You probably could use an anti-mouse secondary antibody with ab66793 since we know it worked well with a Cy3-conjugated secondary antibody. But, there are 7.5 biotin molecules per IgG1 molecule, and that could cause ...

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We could not find the sequence of Macaque monkey HB-EGF in databases. However, since the green monkey HB-EGF sequence has 97% identity to that of human, there is a good chance that ab66793 react with Macaque HB-EGF. Though the human HB-EGF has 80% iden...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"