生物素Anti-HBEGF/DTR抗体[4G10] (ab66793)
Key features and details
- Biotin Mouse monoclonal [4G10] to HBEGF/DTR
- Suitable for: IP, ICC/IF, WB
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG1
Related conjugates and formulations
概述
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产品名称
生物素Anti-HBEGF/DTR抗体[4G10]
参阅全部 HBEGF/DTR 一抗 -
描述
生物素小鼠单克隆抗体[4G10] to HBEGF/DTR -
宿主
Mouse -
偶联物
Biotin -
经测试应用
适用于: IP, ICC/IF, WBmore details -
种属反应性
与反应: Human
不与反应: Mouse -
免疫原
Recombinant fragment corresponding to Human HBEGF/DTR.
Database link: Q99075 -
表位
EGF domain -
阳性对照
- Vero cell extract carrying human HG-EGF expression vector.
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常规说明
Biotin/IgG = 7.5
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 6
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Protein G purified -
纯化说明
Produced in serum-free medium and purified by combination of chromatography. -
克隆
单克隆 -
克隆编号
4G10 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab66793于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IP |
Use at an assay dependent dilution.
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ICC/IF |
Use at an assay dependent dilution.
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WB |
Use a concentration of 0.2 - 1 µg/ml. Detects a band of approximately 20-28 kDa (predicted molecular weight: 23 kDa).
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说明 |
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IP
Use at an assay dependent dilution. |
ICC/IF
Use at an assay dependent dilution. |
WB
Use a concentration of 0.2 - 1 µg/ml. Detects a band of approximately 20-28 kDa (predicted molecular weight: 23 kDa). |
靶标
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功能
May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts and smooth muscle but not endothelial cells. It is able to bind EGF receptors with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor. -
序列相似性
Contains 1 EGF-like domain. -
翻译后修饰
Several N-termini have been identified by direct sequencing. The forms with N-termini 63, 73 and 74 have been tested and found to be biologically active.
O-linked glycan attachment sites were determined by Edman degradation, O-glycanase digest suggests mucin-type glycosylation (done in HB-EGF purified from histiocytic lymphoma cell line U-937). -
细胞定位
Cell membrane and Secreted > extracellular space. Mature HB-EGF is released into the extracellular space and probably binds to a receptor. - Information by UniProt
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数据库链接
- Entrez Gene: 1839 Human
- Omim: 126150 Human
- SwissProt: Q99075 Human
- Unigene: 799 Human
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别名
- diphtheria toxin receptor (heparin-binding EGF-like growth factor) antibody
- diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor) antibody
- Diphtheria toxin receptor antibody
see all
图片
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All lanes : Biotin Anti-HBEGF/DTR antibody [4G10] (ab66793) at 1 µg/ml
Lane 1 : Vero (African green monkey kidney epithelial cell) whole cell lysate
Lane 2 : Vero cell extract from cells carrying human HBEGF/DTR expression vector
Lane 3 : Vero cell extract from cells carrying mouse HBEGF/DTR expression vector
Predicted band size: 23 kDa
Observed band size: 20-28 kDa why is the actual band size different from the predicted?
Additional bands at: 41 kDa. We are unsure as to the identity of these extra bands. -
These images shows Vero cells carrying human HG-EGF expression vector (left hand image) and Vero cells carrying mouse HBEGF/DTR expression vector (right hand image). Cells treated with ab66793, fixed with 4% PFA and reacted with Cys3 conjugated 2nd antibody.
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All lanes : HBEGF/DTR antibody [4G10](biotin) - Azide free (ab66793) at 1 µg/ml. Lane 1 : Vero cell extract Lane 2 : Vero cell extract from cells carrrying human HBEGF/DTR expression vector. Lane 3 : Vero cell extract from cells carrying mouse HBEGF/DTR expression vector Predicted band size : 23 kDa Observed band size : 20-38 kDa Additional bands at : 41 kDa. We are unsure as to the identity of these extra bands. This image shows Immunoprecipitation of HBEGF/DTR followed by Western blotting/SDS PAGE; the cell surface was biotinylated.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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