Anti-HBEGF/DTR抗体[4G10] (ab66792)
Key features and details
- Mouse monoclonal [4G10] to HBEGF/DTR
- Suitable for: IP, WB, ICC/IF
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
Related conjugates and formulations
概述
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产品名称
Anti-HBEGF/DTR抗体[4G10]
参阅全部 HBEGF/DTR 一抗 -
描述
小鼠单克隆抗体[4G10] to HBEGF/DTR -
宿主
Mouse -
经测试应用
适用于: IP, WB, ICC/IFmore details -
种属反应性
与反应: Human, Recombinant fragment -
免疫原
Recombinant fragment corresponding to Human HBEGF/DTR.
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表位
EGF domain -
阳性对照
- Vero cell extract carrrying human HBEGF/DTR expression vector.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Protein G purified -
纯化说明
Produced in serum-free medium and purified by combination of chromatography -
克隆
单克隆 -
克隆编号
4G10 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab66792于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IP |
Use at an assay dependent concentration.
Use at 2µg/ml |
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WB |
Use a concentration of 0.2 - 1 µg/ml. Detects a band of approximately 20-28 kDa (predicted molecular weight: 23, 40 kDa).
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ICC/IF |
Use a concentration of 5 - 10 µg/ml.
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说明 |
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IP
Use at an assay dependent concentration. Use at 2µg/ml |
WB
Use a concentration of 0.2 - 1 µg/ml. Detects a band of approximately 20-28 kDa (predicted molecular weight: 23, 40 kDa). |
ICC/IF
Use a concentration of 5 - 10 µg/ml. |
靶标
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功能
May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts and smooth muscle but not endothelial cells. It is able to bind EGF receptors with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor. -
序列相似性
Contains 1 EGF-like domain. -
翻译后修饰
Several N-termini have been identified by direct sequencing. The forms with N-termini 63, 73 and 74 have been tested and found to be biologically active.
O-linked glycan attachment sites were determined by Edman degradation, O-glycanase digest suggests mucin-type glycosylation (done in HB-EGF purified from histiocytic lymphoma cell line U-937). -
细胞定位
Cell membrane and Secreted > extracellular space. Mature HB-EGF is released into the extracellular space and probably binds to a receptor. - Information by UniProt
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数据库链接
- Entrez Gene: 1839 Human
- Omim: 126150 Human
- SwissProt: Q99075 Human
- Unigene: 799 Human
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别名
- diphtheria toxin receptor (heparin-binding EGF-like growth factor) antibody
- diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor) antibody
- Diphtheria toxin receptor antibody
see all
图片
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All lanes : Anti-HBEGF/DTR antibody [4G10] (ab66792) at 1 µg/ml
Lane 1 : Vero (African green monkey kidney epithelial cell) whole cell lysate
Lane 2 : Vero cell extract from cells carrying human HBEGF/DTR expression vector
Lane 3 : Vero cell extract from cells carrying mouse HBEGF/DTR expression vector
Predicted band size: 23, 40 kDa
Observed band size: 20-28 kDa why is the actual band size different from the predicted?
Additional bands at: 41 kDa. We are unsure as to the identity of these extra bands. -
Lane 1: Vero cell extract, 1μg
Lane 2: Vero cells carrying Human HBEGF/DTR expression vector, 1μg
Lane 3: Vero Cells carrying mouse HBEGF/DTR expression vector, 1μgAb66792 immunoprecipitating HBEGF/DTR in Vero Cells. For western blotting, a HRP conjugated streptavidin was used as the secondary antibody; cell surface was biotinylated.
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These images show Vero cells carrying human HG-EGF expression vector (left hand image) and Vero cells carrying mouse HBEGF/DTR expression vector (right hand image). Cells treated with ab 66792, fixed with 4% PFA and reacted with Cys3 conjugated 2nd antibody.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab66792 被引用在 5 文献中.
- Arnold AE et al. Attenuated diphtheria toxin mediates siRNA delivery. Sci Adv 6:N/A (2020). PubMed: 32917630
- Shen L et al. C-X-C motif chemokine ligand 8 promotes endothelial cell homing via the Akt-signal transducer and activator of transcription pathway to accelerate healing of ischemic and hypoxic skin ulcers. Exp Ther Med 13:3021-3031 (2017). PubMed: 28587375
- Bæk R et al. Does smoking, age or gender affect the protein phenotype of extracellular vesicles in plasma? Transfus Apher Sci 55:44-52 (2016). PubMed: 27470710
- Tang XH et al. The anti-tumor effect of cross-reacting material 197, an inhibitor of heparin-binding EGF-like growth factor, in human resistant ovarian cancer. Biochem Biophys Res Commun 422:676-80 (2012). Human . PubMed: 22609777
- Desjardins F et al. Modulation of the cochaperone AHA1 regulates heat-shock protein 90 and endothelial NO synthase activation by vascular endothelial growth factor. Arterioscler Thromb Vasc Biol 32:2484-92 (2012). PubMed: 22859491