使用敲除细胞株进行验证RabMAb

Anti-HAUSP / USP7抗体[EPR4253] (ab108931)

概述

  • 产品名称Anti-HAUSP / USP7抗体[EPR4253]
    参阅全部 HAUSP / USP7 一抗
  • 描述
    兔单克隆抗体[EPR4253] to HAUSP / USP7
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
    不适用于: Flow Cyt or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human HAUSP/ USP7.

  • 阳性对照
    • Ramos, HeLa, A431, MCF7, C6 and RAW 264.7 cell lysates; Human colon tissue; HeLa cells.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab108931 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 128 kDa.
IHC-P 1/50 - 1/100. Antigen retrieval is recommended.
ICC/IF 1/100 - 1/250.
  • 应用说明Is unsuitable for Flow Cyt or IP.
  • 靶标

    • 功能Hydrolase that deubiquitinates target proteins such as FOXO4, p53/TP53, MDM2, ERCC6, DNMT1, UHRF1, PTEN and DAXX (PubMed:11923872, PubMed:15053880, PubMed:16964248, PubMed:18716620, PubMed:25283148). Together with DAXX, prevents MDM2 self-ubiquitination and enhances the E3 ligase activity of MDM2 towards p53/TP53, thereby promoting p53/TP53 ubiquitination and proteasomal degradation. Deubiquitinates p53/TP53, preventing degradation of p53/TP53, and enhances p53/TP53-dependent transcription regulation, cell growth repression and apoptosis (PubMed:25283148). Deubiquitinates p53/TP53 and MDM2 and strongly stabilizes p53/TP53 even in the presence of excess MDM2, and also induces p53/TP53-dependent cell growth repression and apoptosis. Deubiquitination of FOXO4 in presence of hydrogen peroxide is not dependent on p53/TP53 and inhibits FOXO4-induced transcriptional activity. In association with DAXX, is involved in the deubiquitination and translocation of PTEN from the nucleus to the cytoplasm, both processes that are counteracted by PML. Involved in cell proliferation during early embryonic development. Involved in transcription-coupled nucleotide excision repair (TC-NER) in response to UV damage: recruited to DNA damage sites following interaction with KIAA1530/UVSSA and promotes deubiquitination of ERCC6, preventing UV-induced degradation of ERCC6. Contributes to the overall stabilization and trans-activation capability of the herpesvirus 1 trans-acting transcriptional protein ICP0/VMW110 during HSV-1 infection. Involved in maintenance of DNA methylation via its interaction with UHRF1 and DNMT1: acts by mediating deubiquitination of UHRF1 and DNMT1, preventing their degradation and promoting DNA methylation by DNMT1 (PubMed:21745816). Exhibits a preference towards 'Lys-48'-linked ubiquitin chains. Increases regulatory T-cells (Treg) suppressive capacity by deubiquitinating and stabilizing the transcription factor FOXP3 which is crucial for Treg cell function (PubMed:23973222).
    • 组织特异性Widely expressed. Overexpressed in prostate cancer.
    • 序列相似性Belongs to the peptidase C19 family.
      Contains 1 MATH domain.
      Contains 1 USP domain.
    • 结构域The C-terminus plays a role in its oligomerization.
    • 翻译后修饰Isoform 1: Phosphorylated. Isoform 1 is phosphorylated at positions Ser-18 and Ser-963. Isoform 2: Not phosphorylated.
      Isoform 1: Polyneddylated. Isoform 2: Not Polyneddylated.
      Isoform 1 and isoform 2: Not sumoylated.
      Isoform 1 and isoform 2: Polyubiquitinated by herpesvirus 1 trans-acting transcriptional protein ICP0/VMW110; leading to its subsequent proteasomal degradation. Isoform 1: Ubiquitinated at Lys-869.
    • 细胞定位Nucleus. Cytoplasm. Nucleus, PML body. Present in a minority of ND10 nuclear bodies. Association with ICP0/VMW110 at early times of infection leads to an increased proportion of USP7-containing ND10. Colocalizes with ATXN1 in the nucleus. Colocalized with DAXX in speckled structures. Colocalized with PML and PTEN in promyelocytic leukemia protein (PML) nuclear bodies.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Deubiquitinating enzyme 7 antibody
      • HAUSP antibody
      • Herpes virus associated ubiquitin specific protease antibody
      • Herpesvirus-associated ubiquitin-specific protease antibody
      • TEF 1 antibody
      • tef-1 antibody
      • TEF1 antibody
      • Ubiquitin carboxyl terminal hydrolase 7 antibody
      • Ubiquitin carboxyl-terminal hydrolase 7 antibody
      • Ubiquitin specific peptidase 7 (herpes virus associated) antibody
      • Ubiquitin specific peptidase 7 antibody
      • Ubiquitin specific peptidase 7 herpes virus associated antibody
      • Ubiquitin specific processing protease 7 antibody
      • Ubiquitin specific protease 7 (herpes virus associated) antibody
      • Ubiquitin specific protease 7 antibody
      • Ubiquitin specific protease 7 herpes virus associated antibody
      • Ubiquitin thioesterase 7 antibody
      • Ubiquitin thiolesterase 7 antibody
      • Ubiquitin-specific-processing protease 7 antibody
      • UBP 7 antibody
      • UBP-7 antibody
      • UBP7 antibody
      • UBP7_HUMAN antibody
      • USP 7 antibody
      • usp-7 antibody
      • Usp7 antibody
      • VMW110-ASSOCIATED PROTEIN, 135-KD antibody
      see all

    Anti-HAUSP / USP7 antibody [EPR4253] 图像



    • Predicted band size : 128 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: HAUSP/USP7 knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: MCF7 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab108931 observed at 115 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab108931 was shown to specifically react with HAUSP/USP7 when HAUSP/USP7 knockout samples were used. Wild-type and HAUSP/USP7 knockout samples were subjected to SDS-PAGE. ab108931 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • All lanes : Anti-HAUSP / USP7 antibody [EPR4253] (ab108931) at 1/1000 dilution

      Lane 1 : Ramos cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : A431 cell lysate
      Lane 4 : MCF7 cell lysate
      Lane 5 : C6 cell lysate
      Lane 6 : RAW 264.7 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 128 kDa
    • ab108931, at 1/50, staining HAUSP / USP7 in Human colon tissue by Immunohistochemistry, Paraffin-embedded tissue.
    • ab108931, at 1/100, staining HAUSP / USP7 in HeLa cells by Immunofluorescence.

    Anti-HAUSP / USP7 antibody [EPR4253] (ab108931)参考文献

    ab108931 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Sample Human Cell lysate - whole cell (HAP1 cells)
    Gel Running Conditions Non-reduced Denaturing (4-12 % Bis-Tris gel)
    Loading amount 50 µg
    Specification HAP1 cells
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    提交于 Oct 20 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.05% Triton X100 in PBS
    Username

    Dr. Kirk McManus

    Verified customer

    提交于 Feb 20 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"