Anti-HAUSP / USP7抗体(ab84098)

概述

  • 产品名称Anti-HAUSP / USP7抗体
    参阅全部 HAUSP / USP7 一抗
  • 描述
    兔多克隆抗体to HAUSP / USP7
  • 经测试应用适用于: IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Pig, Rhesus monkey, Zebra finch
  • 免疫原

    Synthetic peptide, corresponding to a region within the amino acids 1-50 of Human HAUSP/ USP7 (accession number: NP_003461.1)

  • 阳性对照
    • Human stomach adenocarcinoma. Mouse squamous cell carcinoma. Human colon adenocarcinoma. Human breast adenocarcinoma.

性能

应用

Our Abpromise guarantee covers the use of ab84098 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/100 - 1/500.
ICC/IF 1/100.

靶标

  • 功能Hydrolase that deubiquitinates target proteins such as FOXO4, p53/TP53, MDM2, ERCC6, DNMT1, UHRF1, PTEN and DAXX (PubMed:11923872, PubMed:15053880, PubMed:16964248, PubMed:18716620, PubMed:25283148). Together with DAXX, prevents MDM2 self-ubiquitination and enhances the E3 ligase activity of MDM2 towards p53/TP53, thereby promoting p53/TP53 ubiquitination and proteasomal degradation. Deubiquitinates p53/TP53, preventing degradation of p53/TP53, and enhances p53/TP53-dependent transcription regulation, cell growth repression and apoptosis (PubMed:25283148). Deubiquitinates p53/TP53 and MDM2 and strongly stabilizes p53/TP53 even in the presence of excess MDM2, and also induces p53/TP53-dependent cell growth repression and apoptosis. Deubiquitination of FOXO4 in presence of hydrogen peroxide is not dependent on p53/TP53 and inhibits FOXO4-induced transcriptional activity. In association with DAXX, is involved in the deubiquitination and translocation of PTEN from the nucleus to the cytoplasm, both processes that are counteracted by PML. Involved in cell proliferation during early embryonic development. Involved in transcription-coupled nucleotide excision repair (TC-NER) in response to UV damage: recruited to DNA damage sites following interaction with KIAA1530/UVSSA and promotes deubiquitination of ERCC6, preventing UV-induced degradation of ERCC6. Contributes to the overall stabilization and trans-activation capability of the herpesvirus 1 trans-acting transcriptional protein ICP0/VMW110 during HSV-1 infection. Involved in maintenance of DNA methylation via its interaction with UHRF1 and DNMT1: acts by mediating deubiquitination of UHRF1 and DNMT1, preventing their degradation and promoting DNA methylation by DNMT1 (PubMed:21745816). Exhibits a preference towards 'Lys-48'-linked ubiquitin chains. Increases regulatory T-cells (Treg) suppressive capacity by deubiquitinating and stabilizing the transcription factor FOXP3 which is crucial for Treg cell function (PubMed:23973222).
  • 组织特异性Widely expressed. Overexpressed in prostate cancer.
  • 序列相似性Belongs to the peptidase C19 family.
    Contains 1 MATH domain.
    Contains 1 USP domain.
  • 结构域The C-terminus plays a role in its oligomerization.
  • 翻译后修饰Isoform 1: Phosphorylated. Isoform 1 is phosphorylated at positions Ser-18 and Ser-963. Isoform 2: Not phosphorylated.
    Isoform 1: Polyneddylated. Isoform 2: Not Polyneddylated.
    Isoform 1 and isoform 2: Not sumoylated.
    Isoform 1 and isoform 2: Polyubiquitinated by herpesvirus 1 trans-acting transcriptional protein ICP0/VMW110; leading to its subsequent proteasomal degradation. Isoform 1: Ubiquitinated at Lys-869.
  • 细胞定位Nucleus. Cytoplasm. Nucleus, PML body. Present in a minority of ND10 nuclear bodies. Association with ICP0/VMW110 at early times of infection leads to an increased proportion of USP7-containing ND10. Colocalizes with ATXN1 in the nucleus. Colocalized with DAXX in speckled structures. Colocalized with PML and PTEN in promyelocytic leukemia protein (PML) nuclear bodies.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Deubiquitinating enzyme 7 antibody
    • HAUSP antibody
    • Herpes virus associated ubiquitin specific protease antibody
    • Herpesvirus-associated ubiquitin-specific protease antibody
    • TEF 1 antibody
    • tef-1 antibody
    • TEF1 antibody
    • Ubiquitin carboxyl terminal hydrolase 7 antibody
    • Ubiquitin carboxyl-terminal hydrolase 7 antibody
    • Ubiquitin specific peptidase 7 (herpes virus associated) antibody
    • Ubiquitin specific peptidase 7 antibody
    • Ubiquitin specific peptidase 7 herpes virus associated antibody
    • Ubiquitin specific processing protease 7 antibody
    • Ubiquitin specific protease 7 (herpes virus associated) antibody
    • Ubiquitin specific protease 7 antibody
    • Ubiquitin specific protease 7 herpes virus associated antibody
    • Ubiquitin thioesterase 7 antibody
    • Ubiquitin thiolesterase 7 antibody
    • Ubiquitin-specific-processing protease 7 antibody
    • UBP 7 antibody
    • UBP-7 antibody
    • UBP7 antibody
    • UBP7_HUMAN antibody
    • USP 7 antibody
    • usp-7 antibody
    • Usp7 antibody
    • VMW110-ASSOCIATED PROTEIN, 135-KD antibody
    see all

Anti-HAUSP / USP7 antibody 图像

  • Detection of Human HAUSP / USP7 by Immunohistochemistry.
    Sample: FFPE section of Human stomach adenocarcinoma.
    Antibody: ab84098 used at a dilution of 1/500.
    Detection: DAB.
  • Detection of Mouse HAUSP / USP7 by Immunohistochemistry.
    Sample: FFPE section of Mouse squamous cell carcinoma.
    Antibody: ab84098 used at a dilution of 1/100.
    Detection: DAB.
  • Detection of Human HAUSP / USP7 by Immunofluorescence.
    Sample: FFPE section of Human colon adenocarcinoma.
    Antibody: ab84098 used at a dilution of 1/100.
    Detection: Green-fluorescent Anti rabbit IgG FITC conjugated, used at a dilution of 1/100.
  • Detection of Human HAUSP / USP7 by Immunofluorescence.
    Sample: FFPE section of Human breast adenocarcinoma.
    Antibody: ab84098 used at a dilution of 1/100.
    Detection: Green-fluorescent Anti rabbit IgG FITC conjugated, used at a dilution of 1/100.

Anti-HAUSP / USP7 antibody (ab84098)参考文献

ab84098 has not yet been referenced specifically in any publications.

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