Anti-HAUS5抗体(ab103721)

概述

  • 产品名称Anti-HAUS5抗体
  • 描述
    兔多克隆抗体to HAUS5
  • 经测试应用适用于: Flow Cyt, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to Human HAUS5 aa 139-167 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • 阳性对照
    • Human colon carcinoma.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明This antibody is purified through a protein A column, followed by peptide affinity purification.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab103721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 相关性HAUS5 is 1 of 8 subunits of the 390-kD Human augmin complex, or HAUS complex. The augmin complex was first identified in Drosophila, and its name comes from the Latin verb 'augmentare,' meaning 'to increase.' The augmin complex is a microtubule-binding complex involved in microtubule generation within the mitotic spindle and is vital to mitotic spindle assembly.
  • 细胞定位Cytoplasm, cytoskeleton, centrosome and spindle.
  • 数据库链接
  • 别名
    • Dgt5 antibody
    • HAUS augmin like complex subunit 5 antibody
    • HAUS augmin like complex, subunit 5 antibody
    • KIAA0841 antibody

Anti-HAUS5 antibody 图像

  • HAUS5 Antibody (N-term) (ab103721) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • ab103721 at 1/50 dilution staining HAUS5 by immunohistochemistry in formalin fixed and paraffin embedded Human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Anti-HAUS5 antibody (ab103721)参考文献

ab103721 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Xenopus laevis Cell (Spindles in X. laevis cytoplasmic egg extract)
Specification Spindles in X. laevis cytoplasmic egg extract
Fixative Methanol
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
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提交于 Oct 05 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Xenopus laevis Cell lysate - other (Xenopus laevis cytoplasmic egg extract)
Loading amount 50 µg
Specification Xenopus laevis cytoplasmic egg extract
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Oct 05 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"