The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.2 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 88 kDa).
Required for normal alignment of chromosomes at metaphase. Phosphorylates histone H3 'Thr-3' during mitosis.
Strongly expressed in testis. Also present in thymus and bone marrow and low levels observed in prostate, intestine, lung, spleen and lymph node. Expressed in fetal skin, liver, kidney and small intestine and also in proliferating but not non-proliferating cell lines.
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Haspin subfamily. Contains 1 protein kinase domain.
Autophosphorylated on both serine and threonine residues (By similarity). Phosphorylated during mitosis. Phosphorylated upon DNA damage, probably by ATM or ATR.
Haploid germ cell-specific nuclear protein kinase antibody
Serine/threonine-protein kinase haspin antibody
Western blot - Haspin antibody (ab21686)
All lanes : Anti-Haspin antibody (ab21686) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg Lane 2 : HeLa Expressing myc-Haspin (interphase) Whole Cell Lysate - Positive Control at 5 µl Lane 3 : HeLa Interphase Whole Cell Lysate - Negative Control at 10 µg Lane 4 : HeLa Expressing myc-Haspin (mitosis) Whole Cell Lysate - Positive Control at 5 µl Lane 5 : HeLa Mitosis Whole Cell Lysate - Negative Control at 10 µg
Secondary All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
Varier RA et al. A phospho/methyl switch at histone H3 regulates TFIID association with mitotic chromosomes. EMBO J29:3967-78 (2010).
Read more (PubMed: 20953165) »
Markaki Y et al. Phosphorylation of histone H3 at Thr3 is part of a combinatorial pattern that marks and configures mitotic chromatin. J Cell Sci122:2809-19 (2009).
Read more (PubMed: 19622635) »