概述

  • 产品名称Anti-HARS抗体[HARSA6]
    参阅全部 HARS 一抗
  • 描述
    小鼠单克隆抗体[HARSA6] to HARS
  • 特异性This antibody reacts with HARS
  • 经测试应用适用于: IHC-P, ICC, IP, Flow Cyt, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    HARS recombinant fragment (Human), containing 50-200 AAs from near the C-terminal.

  • 阳性对照
    • F2408 whole cell lysate, HeLa cells and HeLa whole cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • 纯度Protein G purified
  • 纯化说明Purified through Protein G, from culture supenatant of hybridoma cultured in a meduim containing bovine IgG-depleted (~95%) fetal bovine serum. Filtered through a 0.22µm membrane.
  • 克隆单克隆
  • 克隆编号HARSA6
  • 同种型IgG1
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab50835 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/10.
IP 1/100.
Flow Cyt 1/10. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB 1/100. Detects a band of approximately 50 kDa (predicted molecular weight: 46 kDa).

靶标

  • 组织特异性Brain, heart, liver and kidney.
  • 疾病相关Defects in HARS are a cause of Usher syndrome type 3B (USH3B) [MIM:614504]. USH3B is a syndrome characterized by progressive vision and hearing loss during early childhood. Some patients have the so-called 'Charles Bonnet syndrome,' involving decreased visual acuity and vivid visual hallucinations. USH is a genetically heterogeneous condition characterized by the association of retinitis pigmentosa with sensorineural deafness. Age at onset and differences in auditory and vestibular function distinguish Usher syndrome type 1 (USH1), Usher syndrome type 2 (USH2) and Usher syndrome type 3 (USH3). USH3 is characterized by postlingual, progressive hearing loss, variable vestibular dysfunction, and onset of retinitis pigmentosa symptoms, including nyctalopia, constriction of the visual fields, and loss of central visual acuity, usually by the second decade of life.
  • 序列相似性Belongs to the class-II aminoacyl-tRNA synthetase family.
    Contains 1 WHEP-TRS domain.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • cytoplasmic antibody
    • EC 6.1.1.21 antibody
    • FLJ20491 antibody
    • HARS antibody
    • HisRS antibody
    • Histidine tRNA ligase, cytoplasmic antibody
    • histidine translase antibody
    • Histidine tRNA ligase antibody
    • Histidine--tRNA ligase antibody
    • Histidyl tRNA synthetase antibody
    • Histidyl-tRNA synthetase antibody
    • HRS antibody
    • Human histidyl tRNA synthetase homolog (HO3) mRNA complete cds antibody
    • SYHC_HUMAN antibody
    • USH3B antibody
    see all

Anti-HARS antibody [HARSA6] 图像

  • Anti-HARS antibody [HARSA6] (ab50835) at 1/100 dilution + Hela whole cell lysate at 25 µg

    Secondary
    Mouse IgG antibody at 1/2500 dilution

    Predicted band size : 46 kDa
    Observed band size : 50,100 kDa (why is the actual band size different from the predicted?)
  • ab50835 at a 1/10 dilution staining HARS in Hela cells, using Alexa Fluor®488 Goat Anti-mouse IgG at 1/200 dilution.
  • ab50835 at a 1/10 dilution staining HARS using Alexa Fluor® 488 Goat Anti-mouse IgG at 1/400 dilution.
  • Anti-HARS antibody [HARSA6] (ab50835) at 1/100 dilution + F2408 whole cell lysate at 25 µg

    Secondary
    Mouse IgG antibody at 1/2500 dilution

    Predicted band size : 46 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • IHC image of ab50835 staining in human cervix formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab50835, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-HARS antibody [HARSA6] (ab50835)参考文献

This product has been referenced in:
  • Chen R  et al. Mechanical-stretch of C2C12 myoblasts inhibits expression of Toll-like receptor 3 (TLR3) and of autoantigens associated with inflammatory myopathies. PLoS One 8:e79930 (2013). WB ; Mouse . Read more (PubMed: 24224022) »

See 1 Publication for this product

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