Anti-Haptoglobin抗体[HG-36] (ab13429)

概述

  • 产品名称Anti-Haptoglobin抗体[HG-36]
    参阅全部 Haptoglobin 一抗
  • 描述
    小鼠单克隆抗体[HG-36] to Haptoglobin
  • 特异性This antibody reacts with the three types of human haptoglobin (types 1-1, 2-1, and 2-2). In an ELISA, the antibody recognizes human haptoglobin as the purified antigen, in human serum, when complexed with human hemoglobin or captured by antibodies. In a competitive ELISA it does not cross react with human IgG, transferrin, C-reactive protein, alpha 1 acid glycoprotein, albumin or hemoglobin, nor with bovine, goat or sheep serum. The antibody reacts against native and denatured (non-reduced) human haptoglobin in an immunoblot or dot blot but fails to recognize the different haptoglobin types under reducing conditions.
  • 经测试应用适用于: WB, ELISA, IHC-P, ICC/IF, Dot blotmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length native protein (Purified haptoglobin types 1-1, 2-1, and 2-2 from pooled human plasma).

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 15mM Sodium Azide
    Constituents: Ascites
  • Concentration information loading...
  • 纯度Ascites
  • 克隆单克隆
  • 克隆编号HG-36
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab13429 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Use under non reducing condition.
ELISA 1/4000.
IHC-P Use at an assay dependent concentration. See Abreview.
ICC/IF Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.

靶标

  • 功能As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an Antimicrobial; Antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway.
    Uncleaved haptoglogin, also known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens.
  • 组织特异性Expressed by the liver and secreted in plasma.
  • 疾病相关Anhaptoglobinemia
  • 序列相似性Belongs to the peptidase S1 family.
    Contains 1 peptidase S1 domain.
    Contains 2 Sushi (CCP/SCR) domains.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Binding peptide antibody
    • BP antibody
    • Haptoglobin alpha chain antibody
    • Haptoglobin alpha(1S) beta antibody
    • Haptoglobin alpha(2FS) beta antibody
    • Haptoglobin beta chain antibody
    • Haptoglobin, alpha polypeptide antibody
    • Haptoglobin, beta polypeptide antibody
    • HP antibody
    • HP2 ALPHA2 antibody
    • HP2ALPHA2 antibody
    • HPA1S antibody
    • HPT antibody
    • HPT_HUMAN antibody
    • MGC111141 antibody
    • Zonulin antibody
    see all

Anti-Haptoglobin antibody [HG-36] 图像

  • ab13429 staining Haptoglobin in human bronchoalveolar lavage cells by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in HOPE, blocked for 5 minutes at 25°C, then incubated with ab13429 at a 1/100 for 1 hour at 25°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-mouse polyclonal used at a 1/200 dilution. Counterstained with DAPI.

    See Abreview

  • ab13429 staining Haptoglobin in human lung cancer tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed with HOPE, blocked for 5 minutes at 25°C, then incubated with ab13429 at a 1/100 dilution for 1 hour at 25°C. The secondary used was an undiluted HRP conjugated goat polyclonal.

    See Abreview

  • This image is courtesy of an anonymous Abreview.

    See Abreview

Anti-Haptoglobin antibody [HG-36] (ab13429)参考文献

This product has been referenced in:
  • Tomazic PV  et al. Nasal mucus proteomic changes reflect altered immune responses and epithelial permeability in patients with allergic rhinitis. J Allergy Clin Immunol N/A:N/A (2013). WB ; Human . Read more (PubMed: 24290289) »
  • Abdullah M & Goldmann T Pulmonary haptoglobin (pHp) is part of the surfactant system in the human lung. Diagn Pathol 7:158 (2012). ICC/IF, IHC-P, Immunomicroscopy ; Human . Read more (PubMed: 23164167) »

See all 4 Publications for this product

Product Wall

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Generally antibodies are stable for 1 year at 4C and for 10 years at -20C or -80C. We however recommend storing the antibodies at 4C for short term (1-2 weeks) because some antibodies lose activity if they kept at 4...

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The on-line document contains general information/guidelines for our antibodies for different purifications. Each product has specific storage instructions which can be found on the public website (on-...

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Thank you for your enquiry and your interest in our products.
This antibody is ascitic fluid and the storage buffer contains 15mM sodium azide. You could use PBS for diluting the antibody. We have some tips/recommendations at this site, you may fi...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (Lung, lung cancer and liver)
Loading amount 30 µg
Specification Lung, lung cancer and liver
Gel Running Conditions Reduced Denaturing (4%stacking, 12%resolving)
Blocking step DIG Wash & Block Buffer, Roche as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
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Abcam user community

Verified customer

提交于 Nov 08 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Lung, lung cancer)
Specification Lung, lung cancer
Fixative HOPE (Hepes-glutamic acid buffer mediated Organic solvent Protection Effect Olert et al.,2001 Pathol Res Pract)
Antigen retrieval step None
Permeabilization No
Blocking step Zytomed Systems Blocking Solution as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Nov 03 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (human bronchoalveolar lavage cells mainly macroph)
Specification human bronchoalveolar lavage cells mainly macroph
Fixative HOPE (Hepes-glutamic acid buffer mediated Organic solvent Protection Effect Olert et al.,2001 Pathol Res Pract)
Permeabilization No
Blocking step Zytomed Systems Blocking Solution as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Nov 03 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (liver,spleen)
Specification liver,spleen
Fixative Formaldehyde
Antigen retrieval step Heat mediated
Blocking step H²O²/Methanol as blocking agent for 15 minute(s) · Concentration: 1%
Username

Abcam user community

Verified customer

提交于 Dec 12 2006

Thank you for your enquiry. We have not epitope mapped this antibody. The antibody recognizes the 3 haptoglobin types in ELISA, dot-blot and immunoblotting. The 3 haptoglobin types have an identical beta-chain. It is likely that the antibody reco...

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Thank you for your enquiry. For ab13429, the antibody recognizes human haptoglobin as the purified antigen, in human serum, when complexed with human hemoglobin or captured by antibodies. In a competitive ELISA it does not cross react with human IgG, t...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"