概述

  • 产品名称Anti-Haptoglobin抗体
    参阅全部 Haptoglobin 一抗
  • 描述
    羊多克隆抗体to Haptoglobin
  • 特异性This product gives a single arc when tested by IEP and 2D-IEP against human serum. Identity is confirmed by double diffusion (Ouchterlony) vs human serum and a known anti-human haptoglobin.
  • 经测试应用适用于: Double Immunodiffusion, RID, Immunoelectrophoresismore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Goat, Horse, Cat, Dog, Human, Pig
    不与反应: Chicken, Guinea pig, Cow
  • 免疫原

    Human haptoglobin purified from serum. All Hp types (Hp1-1, Hp1-2 and Hp2-2) are present in the immunogen.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Glycine buffered saline pH7.4, 0.1% Sodium Azide, 0.1% EACA, 0.01% Benzamidine,1mM EDTA
  • Concentration information loading...
  • 纯度IgG fraction
  • 纯化说明Antiserum is prepared by immunisation of sheep with Human Haptoglobin and, if necessary, adsorbed to monospecificity by use of solid-phase adsorbents. An immunoglobulin fraction is then produced. The titre is adjusted so that inter-batch variation is within10%. The product is 0.2µm filtered.
  • 克隆多克隆
  • 同种型IgG
  • 轻链类型unknown
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab8968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Double Immunodiffusion Use at an assay dependent dilution. 10µL antiserum vs 5µL serum
RID Use at an assay dependent dilution. (and Rocket IEP) 2µL antiserum/cm² gel vs 5µL neat – 1/10 dilution human serum.
The use of 3% PEG 6000 with 1.2%agarose in a suitable buffer (such as TBE or Tris-barbital pH >8.2) is recommended. Suitability for use in nephelometry, immunohistochemical procedures, enzyme-linked immunosorbent assays and Western blot has not been assessed but use in such assays should not necessarily be excluded.
Immunoelectrophoresis Use at an assay dependent dilution. 100µL antiserum vs 5µL serum
Rocket Immunoelectrophoresis Use at an assay dependent concentration.

靶标

  • 功能As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an Antimicrobial; Antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway.
    Uncleaved haptoglogin, also known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens.
  • 组织特异性Expressed by the liver and secreted in plasma.
  • 疾病相关Anhaptoglobinemia
  • 序列相似性Belongs to the peptidase S1 family.
    Contains 1 peptidase S1 domain.
    Contains 2 Sushi (CCP/SCR) domains.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Binding peptide antibody
    • BP antibody
    • Haptoglobin alpha chain antibody
    • Haptoglobin alpha(1S) beta antibody
    • Haptoglobin alpha(2FS) beta antibody
    • Haptoglobin beta chain antibody
    • Haptoglobin, alpha polypeptide antibody
    • Haptoglobin, beta polypeptide antibody
    • HP antibody
    • HP2 ALPHA2 antibody
    • HP2ALPHA2 antibody
    • HPA1S antibody
    • HPT antibody
    • HPT_HUMAN antibody
    • MGC111141 antibody
    • Zonulin antibody
    see all

Anti-Haptoglobin antibody (ab8968)参考文献

This product has been referenced in:
  • Williams LM  et al. The development of diet-induced obesity and glucose intolerance in C57BL/6 mice on a high-fat diet consists of distinct phases. PLoS One 9:e106159 (2014). WB ; Mouse . Read more (PubMed: 25170916) »
  • Caperna TJ  et al. Identification and characterization of a nuclear factor-? B-p65 proteolytic fragment in nuclei of porcine hepatocytes in monolayer culture. Domest Anim Endocrinol 45:154-62 (2013). WB ; Pig . Read more (PubMed: 24011531) »

See all 3 Publications for this product

Product Wall

Thank you for your enquiry. All the information we have regarding ab8968 is located on the online datasheet. Ab8968 is 225.00 USD/255.00 Euro. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"