概述

  • 产品名称
    Anti-Haptoglobin抗体
    参阅全部 Haptoglobin 一抗
  • 描述
    兔多克隆抗体to Haptoglobin
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, ELISA, IHC-P, Sandwich ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Human Haptoglobin

  • 阳性对照
    • Human liver tissue

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液
    Preservative: 0.01% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 纯化说明
    ab85846 recognizes Human Haptoglobin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab85846 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 45 kDa.
ELISA 1/3000 - 1/12000.
IHC-P Use a concentration of 2.5 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Sandwich ELISA Use a concentration of 0.5 µg/ml. For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse polyclonal to Haptoglobin (ab52652) as Capture.

靶标

  • 功能
    As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an Antimicrobial; Antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway.
    Uncleaved haptoglogin, also known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens.
  • 组织特异性
    Expressed by the liver and secreted in plasma.
  • 疾病相关
    Anhaptoglobinemia
  • 序列相似性
    Belongs to the peptidase S1 family.
    Contains 1 peptidase S1 domain.
    Contains 2 Sushi (CCP/SCR) domains.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
    • 别名
      • Binding peptide antibody
      • BP antibody
      • Haptoglobin alpha chain antibody
      • Haptoglobin alpha(1S) beta antibody
      • Haptoglobin alpha(2FS) beta antibody
      • Haptoglobin beta chain antibody
      • Haptoglobin, alpha polypeptide antibody
      • Haptoglobin, beta polypeptide antibody
      • HP antibody
      • HP2 ALPHA2 antibody
      • HP2ALPHA2 antibody
      • HPA1S antibody
      • HPT antibody
      • HPT_HUMAN antibody
      • MGC111141 antibody
      • Zonulin antibody
      see all

    图片

    文献

    This product has been referenced in:
    • Solivio MJ  et al. Adsorbing/dissolving Lyoprotectant Matrix Technology for Non-cryogenic Storage of Archival Human Sera. Sci Rep 6:24186 (2016). Read more (PubMed: 27068126) »
    • Tan Y  et al. Acute coronary syndrome remodels the protein cargo and functions of high-density lipoprotein subfractions. PLoS One 9:e94264 (2014). WB ; Human . Read more (PubMed: 24736723) »

    See all 4 Publications for this product

    发表研究结果有使用 ab85846？请让我们知道，以便我们可以引用本数据表中的参考文章。

    客户评价及客户问答

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    1 Review or Q&A

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    Thank you for the note. We will order these antibodies for haptoglobin and free hemoglobin. Wonder if they are good to use for human blood. Do you have standard to control the reaction.

    These two products will be suitable standards:

    Hemoglobin - ab77858 (purified from Human erythrocytes)
    Haptoglobin - ab77872 (purified from Human plasma)

    Please contact us if you have additional questions.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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