The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml.
Use at an assay dependent dilution. PubMed: 12857863
Use at an assay dependent dilution.
Many transcripts for proteins specifically expressed in haploid germ cells are synthesized in the spermatogenesis. A monoclonal antibody TRA54 recognizes specific organellaes in germ cell cytoplasm from spermatocytes to spermatids.
Western blot - Haploid sperm cell-specific antigen antibody [TRA54] (ab92286)
All lanes : Anti-Haploid sperm cell-specific antigen antibody [TRA54] (ab92286) at 1/5000 dilution
Lane 1 : Mouse brain cell lysate Lane 2 : Mouse Heart cell lysate Lane 3 : Mouse Intestine cell lysate Lane 4 : Mouse Kidney cell lysate Lane 5 : Mouse Liver cell lysate Lane 6 : Mouse Muscle cell lysate Lane 7 : Mouse Ovary cell lysate Lane 8 : Mouse Skin cell lysate Lane 9 : Mouse Spleen cell lysate Lane 10 : Mouse Testis cell lysate
Arroteia KF et al. Albumin is synthesized in epididymis and aggregates in a high molecular mass glycoprotein complex involved in sperm-egg fertilization. PLoS One9:e103566 (2014).
Read more (PubMed: 25084016) »
Arrotéia KF et al. Identification and characterization of an antigen recognized by monoclonal antibody TRA 54 in mouse epididymis and vas deferens. J Androl25:914-21 (2004).
Read more (PubMed: 15477363) »