Synthetic peptide conjugated to KLH, corresponding to a region within N terminal amino acids 17-46 of Human HAPLN1.
MCF-7, Jurkat and HepG2 cell lysates; Human prostate carcinoma
Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
Preservative: 0.09% Sodium Azide
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Immunogen affinity purified
ab103455 is purified through a protein A column, followed by peptide affinity purification.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Predicted molecular weight: 40 kDa.
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Stabilizes the aggregates of proteoglycan monomers with hyaluronic acid in the extracellular cartilage matrix.
Widely expressed. Weakly expressed in the brain.
Belongs to the HAPLN family.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. Contains 2 Link domains.
Secreted, extracellular space, extracellular matrix.
Information by UniProt
Cartilage link protein antibody
cartilage linking protein 1 antibody
Cartilage-link protein antibody
Western blot - HAPLN1 antibody (ab103455)
All lanes : Anti-HAPLN1 antibody (ab103455) at 1/100 dilution Lane 1 : MCF-7 cell lysate Lane 2 : Jurkat cell lysate Lane 3 : HepG2 cell lysate Lysates/proteins at 35 µg per lane. Predicted band size: 40 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HAPLN1 antibody (ab103455)
ab103455, at 1/50 dilution, staining HAPLN1 in formalin-fixed, paraffin-embedded Human prostate carcinoma by Immunohistochemistry using a peroxidase conjugated secondary antibody and DAB staining.
This product has been referenced in:
et al. Extracellular Matrix Proteomics Reveals Interplay of Aggrecan and Aggrecanases in Vascular Remodeling of Stented Coronary Arteries. Circulation 137:166-183 (2018).
Read more (PubMed: 29030347) »
et al. Abnormal expression of chondroitin sulphate N-acetylgalactosaminyltransferase 1 and Hapln-1 in cartilage with Kashin-Beck disease and primary osteoarthritis. Int Orthop 37:2051-9 (2013).
Read more (PubMed: 23748413) »
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for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"