This fast track antibody is not yet fully characterized. It is subject to these terms and conditions

Anti-Hairless抗体(ab37540)

概述

性能

应用

Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use

应用 Ab评论 说明
ICC/IF 1/150. This Fast-track antibody has been successfully used for ICC/IF using rat oligodendrocyte cell culture. However, please be aware that this information should be used for information purposes only, and that Abcam cannot guarantee that all batches of this antibody will be equally suitable.
WB Use a concentration of 5 µg/ml. This Fast-track antibody has been successfully used for WB using rat brain tissue lysates. However, please be aware that this information should be used for information purposes only, and that Abcam cannot guarantee that all batches of this antibody will be equally suitable.
ELISA Use at an assay dependent concentration. : This antibody gave a positive result in ELISA against the immunizing peptide (ab37539).
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. This Fast-track antibody has been successfully used for IHC-P on oral mucosa tissue. However, please be aware that this information should be used for information purposes only, and that Abcam cannot guarantee that all batches of this antibody will be equally suitable.

靶标

  • 功能May act as a transcription factor that could act on to regulate one of the phases of hair growth.
  • 组织特异性Strongest expression of isoforms 1 and 2 is seen in the small intestine, weaker expression in brain and colon, and trace expression is found in liver, pancreas, spleen, thymus, stomach, salivary gland, appendix and trachea. Isoform 1 is always the most abundant. Isoform 1 is exclusively expressed at low levels in kidney and testis. Isoform 2 is exclusively expressed at high levels in the skin.
  • 疾病相关Defects in HR are the cause of alopecia universalis congenita (ALUNC) [MIM:203655]. ALUNC is a rare autosomal recessive form of hair loss characterized by hair follicles without hair.
    Defects in HR are the cause of atrichia with papular lesions (APL) [MIM:209500]; also known as congenital atrichia. APL is an autosomal recessive disease characterized by papillary lesions over most of the body and almost complete absence of hair.
    Defects in HR are the cause of hypotrichosis Marie Unna type 1 (MUHH1) [MIM:146550]. An autosomal dominant condition characterized by reduced amount of hair, alopecia, little or no eyebrows, eyelashes or body hair, and coarse, wiry, twisted hair in early childhood.
  • 序列相似性Contains 1 JmjC domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ALUNC antibody
    • AU antibody
    • HAIR_HUMAN antibody
    • Hairless protein antibody
    • Host range antibody
    • HR antibody
    • HSA277165 antibody
    • Protein hairless antibody
    see all

Anti-Hairless antibody (ab37540)参考文献

ab37540 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Rat Tissue lysate - whole (Brain)
Loading amount 30 µg
Specification Brain
Gel Running Conditions Reduced Denaturing (12% Novex Gels)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Oct 11 2011

Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Oligodendrocyte)
Specification Oligodendrocyte
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% triton X-100
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
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提交于 Jul 08 2011

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Oral mucosa)
Specification Oral mucosa
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric buffer, pH6
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
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提交于 Jun 07 2011

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (P19)
Specification P19
Fixative Formaldehyde
Permeabilization Yes - 0.2% Triton X 100
Blocking step Serum as blocking agent for 2 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 26°C
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提交于 Mar 26 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"