Anti-hair cortex Cytokeratin抗体(ab112444)


  • 产品名称Anti-hair cortex Cytokeratin抗体
    参阅全部 hair cortex Cytokeratin 一抗
  • 描述
    兔多克隆抗体to hair cortex Cytokeratin
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Macaque Monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to Human hair cortex Cytokeratin aa 1-100 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q6A162

  • 阳性对照
    • This antibody gave a positive signal in the following Human whole cell lysates: Caco2; MCF7; SW480; LOVO. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal skin.


  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域


Our Abpromise guarantee covers the use of ab112444 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).


  • 功能May play a role in late hair differentiation.
  • 组织特异性Expressed in skin and scalp. Also very weakly expressed in tongue, breast, colon and small intestine. In the hair follicle, it is specifically present in the upper hair cuticle. Not present in the upper cortex (at protein level).
  • 序列相似性Belongs to the intermediate filament family.
  • 发展阶段During differentiation of the hair, it is one of the last keratins expressed.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CK-40 antibody
    • Cytokeratin-40 antibody
    • K1C40_HUMAN antibody
    • K40 antibody
    • Keratin antibody
    • Keratin, type I cytoskeletal 40 antibody
    • Keratin-40 antibody
    • Krt40 antibody
    • type I cytoskeletal 40 antibody
    • Type I hair keratin Ka36 antibody
    see all

Anti-hair cortex Cytokeratin antibody 图像

  • IHC image of hair cortex Cytokeratin staining in Human normal skin formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab112444, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

  • All lanes : Anti-hair cortex Cytokeratin antibody (ab112444) at 1 µg/ml

    Lane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 3 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
    Lane 4 : LOVO (Human colon adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 48 kDa
    Observed band size : 48 kDa

    Exposure time : 2 minutes

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab112444 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

Anti-hair cortex Cytokeratin antibody (ab112444)参考文献

ab112444 has not yet been referenced specifically in any publications.

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