Anti-HADHSC抗体[1A12BC8] (ab110284)


  • 产品名称Anti-HADHSC抗体[1A12BC8]
    参阅全部 HADHSC 一抗
  • 描述
    小鼠单克隆抗体[1A12BC8] to HADHSC
  • 经测试应用适用于: IHC-P, ICC/IF, IP, Flow Cyt, In-Cell ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Human liver mitochondria

  • 阳性对照
    • MRC5 fibroblasts; Human cerebellum tissue; Human liver mitochondria; HL-60 cells.
  • 常规说明

    Product was previously marketed under the MitoSciences sub-brand.



Our Abpromise guarantee covers the use of ab110284 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/250. Perform heat mediated antigen retrieval - 1 min pressure cook in 1mmol EDTA pH8.
ICC/IF Use a concentration of 5 µg/ml.
IP Use at an assay dependent concentration.
Flow Cyt Use a concentration of 1 µg/ml. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
In-Cell ELISA Use a concentration of 2 µg/ml. (0.2 µg/well).


  • 功能Plays an essential role in the mitochondrial beta-oxidation of short chain fatty acids. Exerts it highest activity toward 3-hydroxybutyryl-CoA.
  • 组织特异性Expressed in liver, kidney, pancreas, heart and skeletal muscle.
  • 通路Lipid metabolism; fatty acid beta-oxidation.
  • 疾病相关Defects in HADH are the cause of 3-alpha-hydroxyacyl-CoA dehydrogenase deficiency (HADH deficiency) [MIM:231530]. HADH deficiency is a metabolic disorder with various clinical presentations including hypoglycemia, hepatoencephalopathy, myopathy or cardiomyopathy, and in some cases sudden death.
    Defects in HADH are the cause of familial hyperinsulinemic hypoglycemia type 4 (HHF4) [MIM:609975]; also known as persistent hyperinsulinemic hypoglycemia of infancy (PHHI) or congenital hyperinsulinism. HHF is the most common cause of persistent hypoglycemia in infancy and is due to defective negative feedback regulation of insulin secretion by low glucose levels. It causes nesidioblastosis, a diffuse abnormality of the pancreas in which there is extensive, often disorganized formation of new islets. Unless early and aggressive intervention is undertaken, brain damage from recurrent episodes of hypoglycemia may occur. HHF4 should be easily recognizable by analysis of acylcarnitine species and that this disorder responds well to treatment with diazoxide. It provides the first 'experiment of nature' that links impaired fatty acid oxidation to hyperinsulinism and that provides support for the concept that a lipid signaling pathway is implicated in the control of insulin secretion.
  • 序列相似性Belongs to the 3-hydroxyacyl-CoA dehydrogenase family.
  • 细胞定位Mitochondrion matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 3 hydroxyacyl Coenzyme A dehydrogenase antibody
    • HAD antibody
    • HADH antibody
    • HADH1 antibody
    • HADHSC antibody
    • HADHSC, formerly antibody
    • HADSC, formerly antibody
    • HCDH antibody
    • HCDH_HUMAN antibody
    • HHF4 antibody
    • Hydroxyacyl CoA dehydrogenase antibody
    • Hydroxyacyl-coenzyme A dehydrogenase antibody
    • hydroxyacyl-coenzyme A dehydrogenase, mitochondrial antibody
    • L 3 hydroxyacyl Coenzyme A dehydrogenase short chain antibody
    • M SCHAD antibody
    • Medium and short chain L 3 hydroxyacyl coenzyme A dehydrogenase antibody
    • Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase antibody
    • MGC8392 antibody
    • mitochondrial antibody
    • MSCHAD antibody
    • OTTHUMP00000162626 antibody
    • OTTHUMP00000219688 antibody
    • SCHAD antibody
    • SCHAD, formerly antibody
    • Short chain 3 hydroxyacyl CoA dehydrogenase mitochondrial antibody
    • short chain 3-hydroxyacyl-coa dehydrogenase antibody
    • Short-chain 3-hydroxyacyl-CoA dehydrogenase antibody
    see all

Anti-HADHSC antibody [1A12BC8] 图像

  • MRC5 fibroblasts labeled with ab110284 at 5µg/ml and stained with Texas Red conjugated to goat anti-mouse secondary.
  • HADHSC immunocaptured from Human liver mitochondria using ab110284.
  • HL-60 cells were stained with 1 µg/ml ab110284 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
  • ab110284, at 1/250 dilution, staining HADHSC in formalin-fixed, paraffin-embedded Human cerebellum by Immunohistochemistry. HADHSC immunoactivity is most intense in neuronal cell bodies, most notably in the large Purkinje cells.

Anti-HADHSC antibody [1A12BC8] (ab110284)参考文献

ab110284 has not yet been referenced specifically in any publications.

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Thank you for contacting Abcam. The epitope for ab110284, the anti-HADHSC antibody has not been mapped and so unfortunately I cannot tell you where it will bind on the protein. The antibody has not been tested in mouse, but I did an alignment between t...

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