Anti-HACE1抗体(ab83776)

概述

  • 产品名称Anti-HACE1抗体
    参阅全部 HACE1 一抗
  • 描述
    兔多克隆抗体to HACE1
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within the internal amino acids 792-841 (

    DVSDWIKNTEYTSGYEREDPVIQWFWEVVEDITQEERVLLLQFVTGSSRV

    ) of Human HACE1 (NP_065822).

  • 阳性对照
    • HeLa cell lysate

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab83776 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 102 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre in peptide based assay is 1/1562500.

靶标

Anti-HACE1 antibody 图像

  • Anti-HACE1 antibody (ab83776) at 1 µg/ml (in 5% skim milk / PBS buffer) + HeLa cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 102 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)

Anti-HACE1 antibody (ab83776)参考文献

ab83776 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Lymphoblastoid)
Loading amount 20 µg
Specification Lymphoblastoid
Gel Running Conditions Reduced Denaturing (4-12% BIS TRIS)
Blocking step Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Oct 03 2012

Thank you for contacting us and for your interest in our products. I am sorry to confirm that as far as we are aware, ab83776 Anti-HACE1 antibody has never been tested with samples from mouse. All tested and guaranteed species cross-reactivity ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"