概述

  • 产品名称
    Anti-HA tag抗体[HA-7]
    参阅全部 HA tag 一抗
  • 描述
    小鼠单克隆抗体[HA-7] to HA tag
  • 经测试应用
    适用于: ELISA, ICC/IF, IP, WBmore details
  • 免疫原

    Synthetic peptide:

    YPYDVPDYA

    conjugated to KLH, corresponding to amino acids 98-106 of Influenza A Virus Hemagglutinin HA tag

  • 阳性对照
    • Whole extracts of transfected 293T (human embryonal kidney) cells, expressing HA-tagged fusion protein.
  • 常规说明

    This product was changed from ascites to tissue culture supernatant on 25th October 2016. The following lot(s) is/are from ascites and is still in stock as of 25th October 2016 - GR275412, GR177296. Lot numbers other than GR275412 and GR177296 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    pH: 7.4
    Preservative: 0.097% Sodium azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • 纯度
    Proprietary Purification
  • 纯化说明
    Purified from Tissue culture supernatant”.
  • 克隆
    单克隆
  • 克隆编号
    HA-7
  • 同种型
    IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab49969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.

Use at an assay dependent concentration.

ICC/IF Use a concentration of 1 - 2 µg/ml.
IP Use a concentration of 0.5 - 2 µg/ml.
WB Use a concentration of 0.25 - 1 µg/ml. Predicted molecular weight: 63 kDa.

靶标

  • 相关性
    Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
  • 别名
    • HA epitope tag antibody
    • HA1 antibody
    • HA2 antibody
    • hemagglutinin antibody
    • Hemagglutinin HA1 chain antibody
    • Hemagglutinin HA2 chain antibody
    see all

图片

  • HEK-293 cells overexpressing N-terminal HA tagged fusion protein were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton™ X-100. Fixed cells were stained with 1 μg/mL Anti-HA tag antibody [HA-7] (ab49969). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.

  • ab49969 Immunoprecipitating HA tag in HA tagged protein transfected 293T whole cell lysate. 25µg of cell lysate was incubated with primary antibody (1/2500 in lysis buffer) and matrix (Protein A/G) for 6 hours at 4°C. For western blotting anti-mouse HRP (ab6789) (1/10000) was used to confirm successful immunoprecipation.

    75kD is specific band for HA tagged protein. 120kD, 55kD, 35kD is non specific band for protein A/G bead.

    See Abreview

  • HEK-293 cells overexpressing C-terminal HA tagged fusion protein were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton™ X-100. Fixed cells were stained with 1 μg/mL Anti-HA tag antibody [HA-7] (ab49969). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.

文献

This product has been referenced in:
  • Guo H  et al. The aspirin-induced long non-coding RNA OLA1P2 blocks phosphorylated STAT3 homodimer formation. Genome Biol 17:24 (2016). WB ; Human . Read more (PubMed: 26898989) »
  • Müller AC  et al. Identifying Kinase Substrates via a Heavy ATP Kinase Assay and Quantitative Mass Spectrometry. Sci Rep 6:28107 (2016). Read more (PubMed: 27346722) »

See all 2 Publications for this product

客户评价及客户问答

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (HA-tag/Myc overexpr. MDA-MB-231 cells, inj.)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 6.0 Citric Acid
Permeabilization
Yes - Tween-20
Specification
HA-tag/Myc overexpr. MDA-MB-231 cells, inj.
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 May 23 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Sample
Mouse Cell (MEL (erythroluekemia cell))
Specification
MEL (erythroluekemia cell)
Permeabilization
No
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jul 10 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Sample
Mouse Cell lysate - whole cell (MEL (erythroluekemia cell))
Specification
MEL (erythroluekemia cell)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jul 09 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Immuno-precipitation step
Protein A/G
Sample
Human Cell lysate - whole cell (293T (human kidney cell))
Specification
293T (human kidney cell)
Total protein in input
25 µg
Username

Abcam user community

Verified customer

提交于 Jul 05 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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