概述

  • 产品名称
  • 描述
    鸡多克隆抗体to HA tag
  • 特异性
    ELISA: this antibody diluted at 1:20,000 gave an O.D.= 1.0 in a 15 minute reaction against peptide conjugated to a different carrier than used for anti peptide purification. HRP conjugated goat anti chicken IgG (IgY) was used and TMB was the substrate.
  • 经测试应用
    适用于: ICC/IF, WB, ELISAmore details
  • 免疫原

    YPYDVPDYA (influenza hemagglutinin-HA (epitope) conjugated to KLH.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    PBS, pH 7.2, sodium azide, 0.1%
  • Concentration information loading...
  • 纯度
    IgY fraction
  • 纯化说明
    Antibodies were immunoaffinity purified using the peptide immobilized on a solid support.
  • 克隆
    多克隆
  • 同种型
    unknown
  • 轻链类型
    unknown
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab9111 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent dilution. PubMed: 20943657
WB 1/1000.
ELISA Use at an assay dependent dilution.

靶标

  • 相关性
    Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
  • 别名
    • HA epitope tag antibody
    • HA1 antibody
    • HA2 antibody
    • hemagglutinin antibody
    • Hemagglutinin HA1 chain antibody
    • Hemagglutinin HA2 chain antibody
    see all

Anti-HA tag antibody 图像

  • ab9111 staining HA tag (red) in 293T cells overexpressing KHAmCt-980 protein by Immunocytochemistry/ Immunofluorescence. Nucleoli stained with DAPI. Green is for specific speckle marker.

Anti-HA tag antibody (ab9111)参考文献

This product has been referenced in:
  • Berck ME  et al. The wiring diagram of a glomerular olfactory system. Elife 5:N/A (2016). Read more (PubMed: 27177418) »
  • Voelkel-Meiman K  et al. Separable Crossover-Promoting and Crossover-Constraining Aspects of Zip1 Activity during Budding Yeast Meiosis. PLoS Genet 11:e1005335 (2015). Bacteria . Read more (PubMed: 26114667) »

See all 6 Publications for this product

Product Wall

Unfortunately our expertise is in the field of antibody production and epitope design. We work closely with a range of collaborators to produce fusion or recombinant proteins and therefore are unable to offer the information that you require. We wis...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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